Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae
Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae
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DOI：
10.1096/fj.10-162925
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发表时间：
2010-10-01
期刊：
影响因子：
4.8
通讯作者：
Abe, Keiko
中科院分区：
文献类型：
--
作者：
Ishimaru, Yoshiro;Katano, Yuka;Abe, Keiko
多囊肾病1-样3(PKD1L3)和多囊肾病2-样1(PKD2L1)蛋白在哺乳动物中形成异构体，作为酸味受体发挥作用。在这里，我们通过使用一系列缺失突变体的免疫共沉淀实验证明了PKD1L3和PKD2L1通过它们的跨膜域相互作用，而不是通过螺旋线圈结构域相互作用。缺失关键相互作用区域的缺失突变体不被转运到细胞表面并留在细胞质中，而PKD1L3和PKD2L1蛋白在细胞表面表达。钙成像分析表明，无论是位于PKD2L1胞浆C端的卷曲结构域还是EF-Hand结构域，都不需要对酸性溶液刺激的反应。在PKD1L3(-/-)小鼠中，PKD2L1不定位于味孔，而在野生型小鼠中定位于味孔。总之，这些结果表明，PKD1L3和PKD2L1之间通过它们的跨膜区的相互作用对于环状和叶状乳头的味觉细胞和培养细胞中正确的通道到细胞表面的运输是必不可少的。-Ishimaru，Y.，Katano，Y.，Yamamoto，K.，Akiba，M.，Misaka，T.，Roberts，R.W.，Asakura，T.，Matsunami，H.，Abe，K.PKD1L3和PKD2L1之间的相互作用是PKD2L1在环状和叶状乳头的味觉细胞中定位所必需的。FASE B J.24,4058-4067(2010)。Www.fasebj.org
The polycystic kidney disease 1-like 3 (PKD1L3) and polycystic kidney disease 2-like 1 (PKD2L1) proteins have been proposed to form heteromers that function as sour taste receptors in mammals. Here, we show that PKD1L3 and PKD2L1 interact through their transmembrane domains, and not through the coiled-coil domain, by coimmunoprecipitation experiments using a series of deletion mutants. Deletion mutants lacking the critical interaction region were not transported to the cell surface and remained in the cytoplasm, whereas PKD1L3 and PKD2L1 proteins were expressed at the cell surface when both are transfected. Calcium imaging analysis revealed that neither the coiled-coil domain nor the EF-hand domain located in the C-terminal cytoplasmic tail of PKD2L1 was required for response on stimulation with an acidic solution. Finally, PKD2L1 did not localize to the taste pore but was distributed throughout the cytoplasm in taste cells of circumvallate and foliate papillae in PKD1L3(-/-) mice, whereas it localized to the taste pore in wild-type mice. Collectively, these results suggest that the interaction between PKD1L3 and PKD2L1 through their transmembrane domains is essential for proper trafficking of the channels to the cell surface in taste cells of circumvallate and foliate papillae and in cultured cells.-Ishimaru, Y., Katano, Y., Yamamoto, K., Akiba, M., Misaka, T., Roberts, R. W., Asakura, T., Matsunami, H., Abe, K. Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae. FASEB J. 24, 4058-4067 (2010). www.fasebj.org