MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimental Echinococcus granulosus infection, using a high-throughput approach.
MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimental Echinococcus granulosus infection, using a high-throughput approach.
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使用高通量方法对实验性细粒棘球绦虫感染后的哈萨克羊肠道组织进行 MicroRNA 分析。
DOI：
10.1051/parasite/2016023
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jia B
中科院分区：
文献类型：
--
作者：
Jiang S;Li X;Wang X;Ban Q;Hui W;Jia B
囊状棘球蚴病是由细粒棘球绦虫幼虫感染引起的一种慢性人畜共患病，绵羊对该病高度易感。在此之前，我们发现具有不同MHC单倍型的哈萨克羊在CE感染上存在差异。具有单倍型MHCMvaIbc-SacIIab-Hin 1 Iab的绵羊对CE感染具有抗性，而没有该单倍型的绵羊则不具有抗性。MicroRNA（miRNAs）是一类非编码小RNA，是转录后水平基因表达的关键调控因子，在发育和代谢等基本生物学过程中发挥重要作用。为了在感染早期鉴定控制对CE抗性的microRNA，在经口感染E.颗粒虫卵在耐药组中共检测到351种已知的和186种新的miRNA，而在非耐药组中检测到353种已知的和129种新的miRNA。在这些miRNAs中，有83个已知的miRNAs表达差异显著，其中75个上调，8个下调。在这些已知的microRNA中，与CE非抗性文库相比，miR-21- 3 p、miR-542- 5 p、miR-671、miR-134- 5 p、miR-26 b和miR-27 a在CE抗性绵羊中显示出显著更高的表达，其中FC > 3。功能分析表明它们是NF-κ B通路应答性miRNAs，参与炎症过程。结果提示，这些microRNA可能在肠组织对大肠杆菌的反应中起重要作用。颗粒状。
Cystic echinococcosis (CE), caused by infection with the larval stage of the cestode Echinococcus granulosus, is a chronic zoonosis, to which sheep are highly susceptible. Previously, we found that Kazakh sheep with different MHC haplotypes differed in CE infection. Sheep with haplotype MHCMvaIbc-SacIIab-Hin1Iab were resistant to CE infection, while their counterparts without this haplotype were not. MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at the post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. To identify microRNA controlling resistance to CE in the early stage of infection, microRNA profiling was conducted in the intestinal tissue of sheep with resistant and non-resistant MHC haplotypes after peroral infection with E. granulosus eggs. A total of 351 known and 186 novel miRNAs were detected in the resistant group, against 353 known and 129 novel miRNAs in the non-resistant group. Among these miRNAs, 83 known miRNAs were significantly differentially expressed, including 75 up-regulated and 8 down-regulated miRNAs. Among these known microRNAs, miR-21-3p, miR-542-5p, miR-671, miR-134-5p, miR-26b, and miR-27a showed a significantly higher expression in CE-resistant sheep compared to the CE-non-resistant library, with the FC > 3. Functional analysis showed that they were NF-kB pathway-responsive miRNAs, which are involved in the inflammation process. The results suggest that these microRNAs may play important roles in the response of intestinal tissue to E. granulosus.