Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.
Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.
复制标题
DOI：
10.1073/pnas.86.21.8299
复制
发表时间：
1989-11
影响因子：
11.1
通讯作者：
Rosa Maria;Del;Angel;Athanasios G. PAPAVASSILIOUt;Carlos FERNANDEZ-TOMAS;Saul J. SILVERSTEINt;
中科院分区：
文献类型：
--
作者：
Rosa Maria;Del;Angel;Athanasios G. PAPAVASSILIOUt;Carlos FERNANDEZ-TOMAS;Saul J. SILVERSTEINt;
脊髓灰质炎病毒RNA的5'非编码区包含翻译和复制所必需的序列。这些功能可能是通过细胞和/或病毒蛋白对脊髓灰质炎病毒RNA的识别来实现的。利用迁移位移电泳和1,10-菲罗啉/Cu+足迹，我们证明了细胞质因子与5'非翻译区（UTR）内核苷酸510-629序列的特异性结合。在5' UTR内还观察到第二个序列（核苷酸97-182）形成复合物。通过竞争分析和离子强度对复合物形成的影响，5' UTR的这两个区域似乎被不同的细胞因子所识别。然而，这两种复合物都含有真核起始因子2 α，这是它们与特异性抗体反应的结果。
The 5' noncoding region of poliovirus RNA contains sequences necessary for translation and replication. These functions are probably carried out by recognition of poliovirus RNA by cellular and/or viral proteins. Using a mobility-shift electrophoresis assay and 1,10-phenanthroline/Cu+ footprinting, we demonstrate specific binding of cytoplasmic factors with a sequence from nucleotides 510-629 within the 5' untranslated region (UTR). Complex formation was also observed with a second sequence (nucleotides 97-182) within the 5' UTR. These two regions of the 5' UTR appear to be recognized by distinct cell factors as determined by competition analysis and the effects of ionic strength on complex formation. However, both complexes contain eukaryotic initiation factor 2 alpha, as revealed by their reaction with specific antibody.