Genomic structure of NKG5, a human NK and T cell-specific activation gene.
Genomic structure of NKG5, a human NK and T cell-specific activation gene.
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NKG5（人类 NK 和 T 细胞特异性激活基因）的基因组结构。
DOI：
10.1007/bf00216832
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发表时间：
1993
期刊：
影响因子：
3.2
通讯作者：
Bach,FH
中科院分区：
文献类型：
--
作者：
Houchins,JP;Kricek,F;Chujor,CS;Heise,CP;Yabe,T;McSherry,C;Bach,FH
我们先前报道了一个cDNA克隆的分离，命名为NKG 5，编码一种分泌蛋白，仅在自然杀伤细胞和T细胞中表达，并在细胞活化后强烈上调。本研究从人胎盘基因组文库中分离出NKG 5基因，并对该基因及其5 ′-侧翼DNA的两个内切酶进行了测序。与cDNA序列比较，NKG 5基因由5个外显子和4个内含子组成。内含子1含有一段DNA片段，据报道它作为外显子出现在519中，519是从T细胞文库中分离的一个密切相关的cDNA克隆。这一结果表明NKG 5和519是单一基因的选择性剪接产物。分析NKG 5基因5′侧翼区与细胞因子和其他活化诱导基因启动子区的同源性，这些基因显示淋巴细胞特异性表达。鉴定了显示序列相似性的几个片段。我们还鉴定了许多序列元件，其与转录调节蛋白（包括T细胞特异性和活化特异性调节因子）的已知结合位点具有很强的相似性。这些发现与观察到的NKG 5基因的细胞特异性表达和严格的调控控制一致。
We reported previously the isolation of a cDNA clone, designatedNKG5, encoding a secreted protein that is expressed only in natural killer and T cells and is strongly upregulated upon cell activation. In this report we have isolated theNKG5gene from a human placental genomic library and sequenced the gene and two kilobases of 5'-flanking DNA. Comparison with the cDNA sequence reveals that theNKG5gene consists of five exons and four introns. Intron 1 contains a DNA segment that was reported to occur as an exon in519, a closely related cDNA clone that was isolated from a T-cell library. This result indicates that NKG5 and 519 are alternative splicing products of a single gene. The 5′-flanking region of theNKG5gene was analyzed for homology with the promoter regions of cytokines and other activation-induced genes showing lymphocyte-specific expression. Several segments displaying sequence similarity were identified. We also identified numerous sequence elements that have strong similarity to known binding sites for transcriptional regulatory proteins including T-cell-specific and activation-specific regulatory factors. These findings are consistent with the cell-specific expression and the tight regulatory control that is observed for theNKG5gene.