1H-1H dipolar couplings provide a unique probe of RNA backbone structure.
1H-1H dipolar couplings provide a unique probe of RNA backbone structure.
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1H-1H 偶极耦合提供了 RNA 主链结构的独特探针。
DOI：
10.1021/ja0388212
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发表时间：
2003
影响因子：
15
通讯作者：
Bax,Ad
中科院分区：
文献类型：
--
作者：
Miclet,Emeric;O'Neil-Cabello,Erin;Nikonowicz,EdwardP;Live,David;Bax,Ad
描述了一种NMR方法，该方法允许同时测量亚甲基的2 JH 1H 2 + 2DH 1H 2分裂和1 JCH 1 + 1DCH 1 + 1 JCH 2 + 1DCH 2耦合的总和，其中2DH 1H 2和1DCH 2是残余偶极耦合，当分子相对于磁场弱取向时发生。通过抑制1H −1H孪态双峰的上半场或下半场，相对于常规的二维1H − 13 C相关谱，实验产生了更高的分辨率，使其适用于相当复杂的系统。该方法被证明用于测量所有2DH 5 'H5''耦合在24-nucleotide 13 C-富集RNA茎环结构，弱排列在液晶Pf 1。该方法同样适用于亚甲基13 C标记的蛋白质和自然丰度的小分子样品。
A NMR method is described that permits simultaneous measurement of the geminal2JH1H2+2DH1H2splitting and the sum of the1JCH1+1DCH1+1JCH2+1DCH2couplings for methylene groups, where2DH1H2and1DCHare residual dipolar couplings, occurring when molecules are weakly oriented relative to the magnetic field. By suppressing either the upfield or downfield half of the1H−1H geminal doublet, the experiment yields improved resolution relative to regular two-dimensional1H−13C correlation spectra, making it applicable to systems of considerable complexity. The method is demonstrated for measurement of all2DH5‘H5‘‘couplings in a 24-nucleotide13C-enriched RNA stem loop structure, weakly aligned in liquid crystalline Pf1. The method is equally applicable to methylene groups in13C-labeled proteins and to natural abundance samples of smaller molecules.