Induction of impaired activation of lymphocytes by suppressive factor in Crohn's disease patients.
Induction of impaired activation of lymphocytes by suppressive factor in Crohn's disease patients.
复制标题
克罗恩病患者中抑制因子诱导淋巴细胞活化受损。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
M. Tsuchiya
中科院分区：
文献类型：
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作者：
M. Watanabe;S. Tsuru;S. Aiso;T. Hibi;T. Yoshida;H. Asakura;Y. Zinnaka;M. Tsuchiya
本文研究了12例克罗恩病（CD）患者淋巴细胞对植物血凝素（PHA）的增殖反应及其与血清因子的关系。克罗恩病患者淋巴细胞增殖反应明显降低（S.I. = 38.8 +/- 36.8）（平均值+/- SD），与正常对照组（S.I. = 100.6 +/- 28.6）（p <0.01）。此外，CD患者或10例溃疡性结肠炎（UC）患者的血清中有非常受损的淋巴细胞对PHA的反应正常淋巴细胞对PHA的增殖反应的影响也进行了测量。CD患者血清对正常淋巴细胞的母细胞生成有明显的抑制作用（S.I. = 46.4 ± 28.5），与正常血清（S.I. = 126.2 +/- 14.7）（p小于0.001）。另一方面，UC血清不抑制正常淋巴细胞的胚细胞生成（S.I. = 114.9 +/- 27.7）。采用单向免疫扩散法测定患者血清免疫抑制酸性蛋白（IAP）水平。与正常对照组（376 +/- 92 μ g/ml）相比，CD患者（780 +/- 470 μ g/ml）和UC患者（601 +/- 278 μ g/ml）的血清IAP水平显著升高（p <0.001）。但CD患者血清IAP水平与血清抑制作用之间无明显相关性。因此，我们证明了CD患者的淋巴细胞对PHA的反应性受损，并且可能存在与CD患者血清中的IAP不相同的免疫抑制因子。
Lymphocyte proliferative response to phytohemagglutinin (PHA) and relevance of serum factors to the response were studied in 12 patients with Crohn's disease (CD). The lymphocyte proliferative response was markedly reduced in patients with Crohn's disease (S.I. = 38.8 +/- 36.8) (mean +/- SD), as compared with normal controls (S.I. = 100.6 +/- 28.6) (p less than 0.01). In addition, the effect of sera from patients with CD or 10 patients with ulcerative colitis (UC) who had extremely impaired lymphocyte responsiveness to PHA on the proliferative response of normal lymphocytes to PHA was also measured. Sera from CD patients had a marked suppressive effect on the blastogenesis of normal lymphocytes (S.I. = 46.4 +/- 28.5), as compared with normal sera (S.I. = 126.2 +/- 14.7) (p less than 0.001). On the other hand, UC sera did not suppress the blastogenesis of normal lymphocytes (S.I. = 114.9 +/- 27.7). Moreover, serum immunosuppressive acidic protein (IAP) levels in patients' sera were measured by single radial immunodiffusion assay. A marked increase in serum IAP levels was revealed both in CD patients (780 +/- 470 micrograms/ml) and in UC patients (601 +/- 278 micrograms/ml), as compared with normal controls (376 +/- 92 micrograms/ml) (p less than 0.001). But there was no precise correlation between the suppressive effect of sera and serum IAP levels in patients with CD. Thus, we demonstrated an impairment of the lymphocyte responsiveness to PHA in CD patients and the possible existence of immunosuppressive factors which is not identical with IAP in the sera from patients with CD.