RAD51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca2
RAD51 interacts with the evolutionarily conserved BRC motifs in the human breast cancer susceptibility gene brca2
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DOI：
10.1074/jbc.272.51.31941
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发表时间：
1997-12-19
影响因子：
4.8
通讯作者：
Bartel, PL
中科院分区：
文献类型：
--
作者：
Wong, AKC;Pero, R;Bartel, PL
最近的工作表明，小鼠BRCA 2肿瘤抑制蛋白与小鼠RAD 51蛋白相互作用，这种相互作用表明BRCA 2参与DNA修复。鼠BRCA 2蛋白的残基3196-3232被证明参与这种相互作用。在这里，我们报告了这两种蛋白质的人类同源物之间相互作用所涉及的其他结构域的详细映射。通过酵母双杂交和生化分析，我们表明，RAD 51蛋白相互作用的8个进化上保守的BRC图案编码的外显子11的brca 2和一个类似的图案中发现的秀丽隐杆线虫假设的蛋白质。缺失分析表明，人RAD 51的98-339位残基与在所有BRC基序中保守的59个残基的最小区域相互作用。这些数据表明BRC重复序列具有结合RAD 51的功能。
Recent work has shown that the murine BRCA2 tumor suppressor protein interacts with the murine RAD51 protein, This interaction suggests that BRCA2 participates in DNA repair. Residues 3196-3232 of the murine BRCA2 protein were shown to be involved in this interaction. Here, we report the detailed mapping of additional domains that are involved in interactions between the human homologs of these two proteins. Through yeast two-hybrid and biochemical assays, we demonstrate that the RAD51 protein interacts specifically with the eight evolutionarily conserved BRC motifs encoded in exon 11 of brca2 and with a similar motif found in a Caenorhabditis elegans hypothetical protein. Deletion analysis demonstrates that residues 98-339 of human RAD51 interact with the 59-residue minimal region that is conserved in all BRC motifs. These data suggest that the BRC repeats function to bind RAD51.