Platelet Administration Via the Portal Vein Promotes Liver Regeneration in Rats After 70% Hepatectomy
Platelet Administration Via the Portal Vein Promotes Liver Regeneration in Rats After 70% Hepatectomy
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DOI：
10.1097/sla.0b013e318211caf8
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发表时间：
2011-04-01
影响因子：
9
通讯作者：
Ohkohchi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Matsuo, Ryota;Nakano, Yoritaka;Ohkohchi, Nobuhiro
目的：探讨血小板输注治疗肝再生的体内适用性。背景：我们最近报道了血小板在大范围肝切除术后立即在肝脏中积聚并促进残肝再生。肝脏再生取决于窦状窦中血小板的累积数量。方法：雄性Sprague-Dawley大鼠肝切除70%，然后分为两组，分别通过门静脉注入1 mL生理盐水（NS）中富血小板血浆（PRP; 1 × 10(9)个血小板/mL）或NS。然后我们分析了肝脏再生和与肝脏再生和功能相关的信号通路。观察肝切除术前后经门静脉输注血小板的动态变化。结果：70%肝切除术后PRP组肝/体重比显著高于NS组，Ki-67标记指数显著高于NS组。Akt通路在PRP中比NS组更早被激活，同时ERK1/2通路被激活，但在PRP组中被延长。经门静脉输注的血小板在70%肝切除术后在窦内积聚，血清肝功能检查和组织学检查显示门静脉输注未引起肝损伤。结论：经门静脉输注血小板促进70%肝切除后无肝损伤大鼠肝再生。这些发现表明，PRP的施用可能是肝再生治疗的一个有用的部分。
Objective: This study examines the applicability of platelet infusion therapy for liver regeneration in vivo.Background: We recently reported that platelets accumulate in the liver immediately after extended hepatectomy and promote residual liver regeneration. Liver regeneration depends on the number of accumulated platelets in the sinusoids.Methods: Male Sprague-Dawley rats underwent 70% hepatectomy and were then assigned to groups that were infused with 1 mL of either platelet-rich plasma (PRP; 1 x 10(9) platelets/mL) in normal saline (NS) or NS via the portal vein. We then analyzed liver regeneration and the signaling pathways that are related to liver regeneration and function. The dynamics of platelets infused via the portal vein were visualized before and after hepatectomy.Results: The liver/body weight ratio after 70% hepatectomy was significantly higher and the Ki-67 labeling index was higher in the PRP, than in the NS group. The Akt pathway was activated earlier in the PRP, than in the NS group with concurrent ERK1/2 pathway activation, but this was prolonged in the PRP group. Many more platelets infused via the portal vein accumulated in the sinusoid after 70% hepatectomy, and serum liver function tests and histological findings revealed that portal infusion did not cause liver damage.Conclusions: Platelets infused via the portal vein promoted liver regeneration after 70% hepatectomy in rats without liver damage. These findings indicate that PRP administration could be a useful part of liver regeneration therapy.