Ordered flow of secretion from accessory glands to specific layers of the spermatophore of mealworm beetles: demonstration with a monoclonal antibody.
Ordered flow of secretion from accessory glands to specific layers of the spermatophore of mealworm beetles: demonstration with a monoclonal antibody.
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从副腺到黄粉虫精囊特定层的有序分泌流：用单克隆抗体演示。
DOI：
10.1002/jez.1402400214
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Happ,GM
中科院分区：
文献类型：
--
作者：
Grimnes,KA;Bricker,CS;Happ,GM
针对雄性黄粉虫甲虫（黄粉虫）豆形附属腺（BAG）的分泌产物制备单克隆抗体。来自一个克隆（PL 6.3）的抗体识别在BAG匀浆中发现的pI为6.6的9，600道尔顿蛋白。PL 6.3抗原首先在2天成虫的BAG蛋白的蛋白质印迹中检测到，并且在接下来的6天内含量增加，直到实现生殖成熟。该抗体还识别一种分子量约为5，000道尔顿的多肽，我们认为该多肽来源于较大的9，600道尔顿抗原。通过使用光镜免疫组织化学，我们定位PL 6.3识别的抗原在细胞类型7（强染色）和细胞类型5（弱染色）。电镜免疫细胞化学结果表明，PL 6.3抗原主要集中在这两种细胞的分泌颗粒中，而在其它细胞中均不存在，PL 6.3抗原从BAG进入其分泌产物，然后进入射精管的前精细胞团。抗原没有随机地与副腺的其他分泌产物混合。当它从袋流入射精管时，它保持在一个连贯的，精确定位的肿块中。在定形精荚内，PL 6.3抗原集中在排列在内腔的离散材料层中。
Monoclonal antibodies were produced against the secretory product of the bean‐shaped accessory gland (BAG) of male mealworm beetles (Tenebrio molitor). Antibodies from one clone (PL 6.3) recognized a 9,600 dalton protein with a pI of 6.6 which was found in homogenates of the BAG. The PL 6.3 antigen was first detected on Western blots of BAG proteins from 2‐day adults, and amounts increased for the next 6 days until reproductive maturation was achieved. The antibody also recognized a polypeptide with a molecular weight (mw) of about 5,000 daltons which we believe to be derived from the larger 9,600 dalton antigen.There are eight types of secretory cells in the BAG. By using light microscopic immunohistochemistry, we localized the antigens recognized by PL 6.3 in cell type 7 (intense staining) and cell type 5 (weak staining). Results from electron microscopic immunocytochemistry showed that antigen PL 6.3 was concentrated in the secretory granules characteristic of each of these two cell types and was absent in all other cell types.PL 6.3 antigens were traced from the BAG into its secretory product and then into the prespermatophoric mass in the ejaculatory duct. The antigen was not randomly mixed with other secretory products of the accessory glands. As it flowed from the BAG and into the ejaculatory duct, it remained in a coherent, precisely localized mass. Within the definitive spermatophore, the PL 6.3 antigen was concentrated in discrete layers of material that line the lumen.