MORPHOMETRIC IMMUNOCHEMICAL ANALYSIS OF NEURONS IN THE NUCLEUS BASALIS OF MEYNERT IN ALZHEIMERS-DISEASE
MORPHOMETRIC IMMUNOCHEMICAL ANALYSIS OF NEURONS IN THE NUCLEUS BASALIS OF MEYNERT IN ALZHEIMERS-DISEASE
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DOI：
10.1016/0006-8993(88)90827-x
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发表时间：
1988-06-28
期刊：
影响因子：
2.9
通讯作者：
WILCOCK, GK
中科院分区：
文献类型：
--
作者：
ALLEN, SJ;DAWBARN, D;WILCOCK, GK
据报道，在阿尔茨海默病中，Meynert的胆碱能基底核中的大细胞丢失。然而，有人提出，在阿尔茨海默病的基底核中可能存在萎缩的神经元，因此难以从大小上与神经胶质细胞区分。这有重要的治疗意义，我们试图澄清的情况下，使用神经元特异性抗血清针对神经元特异性烯醇化酶（NSE）。使用该抗血清对基底核切片进行染色，并测量神经元横截面积。通过图像分析获得了神经元分布与面积的关系，并在对照组和阿尔茨海默病患者中进行了比较。在阿尔茨海默氏病中发现了显著的29%的总体神经元损失，其中大神经元损失更大（61%），同时小神经元增加（59%）。方差分析显示，作为这种频率向小细胞优势的转变的结果，平均横截神经元面积显着减少。这表明，在阿尔茨海默氏病的基底核，大神经元并没有完全丢失，许多是萎缩，从而排除了以前的研究中的大细胞计数的Nissl染色材料。这些神经元的部分保存使得阿尔茨海默病的特征性胆碱能功能障碍更可能受到神经营养影响。
In Alzeheimer''s disease there is a reported loss of large cells in the cholinergic nucleus basalis of Meynert. It has been suggested, however, that there may be neurons in the nucleus basalis in Alzeheimer''s disease which are atrophied and therefore difficult to distinguish from neuroglia by size. This has important therapeutic implications and we have attempted to clarify the situation using a neuron-specific antiserum directed against neuron-specific enolase (NSE). Sections of nucleus basalis were stained using this antiserum and the neuronal cross-sectional area was measured. A profile of neuronal distribution with area was obtained, by image analysis, and compared in controls and patients with Alzheimer''s disease. A significant 29% overall loss neurons was found in Alzheimer''s disease with a much greater loss (61%) of large neurons and concurrent increase (59%) in small neurons. Analysis of variance showed significant reduction in mean cross-sectional neuronal area as a consequence of this shift in frequency towards a preponderance of small cells. It is suggested that in the nucleus basalis in Alzheimer''s disease, large neurons are not completely lost; many are shrunken and thus excluded from the previous studies of large cells counted in Nissl-stained material. That there is partial preservation of these neurons makes it more likely that cholinergic dysfunction, characteristic of Alzheimer''s disease, will be amenable to neurotrophic influence.