Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors
Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors
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DOI：
10.1016/j.bmcl.2008.05.040
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发表时间：
2008-07-01
影响因子：
2.7
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
--
作者：
Kawaguchi, Mitsuyasu;Terai, Takuya;Nagano, Tetsuo
We have developed a fluorescently labeled probe for high-throughput screening of kinase inhibitors using fluorescence correlation spectroscopy. With this probe, we have successfully evaluated the inhibitory activities of known inhibitors of a model kinase, ASK1. Because the probe contains a general kinase inhibitor, staurosporine, we believe that this homogeneous, high-throughput, and simple method can be applied to the inhibitor screening of other kinases as well. (C) 2008 Elsevier Ltd. All rights reserved.
