OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression
OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression
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DOI：
10.1046/j.1365-313x.2003.01661.x
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发表时间：
2003-02-01
期刊：
影响因子：
7.2
通讯作者：
Yamaguchi-Shinozaki, K
中科院分区：
文献类型：
--
作者：
Dubouzet, JG;Sakuma, Y;Yamaguchi-Shinozaki, K
转录因子DREB/CBF与脱水反应元件/C重复序列（DRE/CRT）顺式作用元件（核心基序：G/ACCGAC）特异性相互作用，控制拟南芥中许多胁迫诱导基因的表达。在水稻中，我们分离了5个DREB同源物的cDNA：OsDREB 1A，OsDREB 1B，OsDREB 1C，OsDREB 1D和OsDREB 2A。OsDREB 1A和OsDREB 1B的表达受冷诱导，而OsDREB 2A的表达受脱水和高盐胁迫诱导。OsDREB 1A和OsDREB 2A蛋白特异性结合DRE，并激活DRE驱动的GUS报告基因在水稻原生质体中的转录。OsDREB 1A在转基因拟南芥中的过量表达诱导了拟南芥DREB 1A的胁迫诱导基因的过量表达，从而使植物对干旱、高盐和冷冻胁迫具有更高的耐受性。这表明OsDREB 1A与DREB 1A具有相似的功能。然而，在微阵列和RNA印迹分析中，DREB 1A蛋白的一些胁迫诱导的靶基因，只有ACCGAC作为DRE在OsDREB 1A转基因拟南芥中没有过表达。OsDREB 1A蛋白与GCCGAC的结合优先于与ACCGAC的结合，而DREB 1A蛋白与GCCGAC和ACCGAC均有效结合。与双子叶植物相比，单子叶植物DREB 1型ERF/AP 2结构域的结构密切相关。OsDREB 1A基因可用于生产耐旱、耐高盐和/或耐冷胁迫的转基因单子叶植物。
The transcription factors DREBs/CBFs specifically interact with the dehydration-responsive element/C-repeat (DRE/CRT) cis -acting element (core motif: G/ACCGAC) and control the expression of many stress-inducible genes in Arabidopsis . In rice, we isolated five cDNAs for DREB homologs: OsDREB1A , OsDREB1B , OsDREB1C , OsDREB1D , and OsDREB2A . Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high-salt stresses. The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Over-expression of OsDREB1A in transgenic Arabidopsis induced over-expression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought, high-salt, and freezing stresses. This indicated that OsDREB1A has functional similarity to DREB1A. However, in microarray and RNA blot analyses, some stress-inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDREB1A transgenic Arabidopsis . The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently. The structures of DREB1-type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots. OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses.