Localization and induction by dehydration of ClC-K chloride channels in the rat kidney.
Localization and induction by dehydration of ClC-K chloride channels in the rat kidney.
复制标题
大鼠肾脏中 ClC-K 氯离子通道脱水的定位和诱导。
DOI：
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发表时间：
1997
影响因子：
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通讯作者：
T. J. Jentsch
中科院分区：
文献类型：
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作者：
Alain Vandewalle;F. Cluzeaud;M. Bens;S. Kieferle;K. Steinmeyer;T. J. Jentsch
我们通过对大鼠肾脏的单个显微解剖肾小管进行逆转录酶-聚合酶链反应，并使用识别这两个高度同源通道的多克隆抗体进行免疫组织化学，研究了两个最近克隆的氯离子通道 rClC-K1 和 rClC-K2 的肾内表达。 rClC-K1 和 rClC-K2 mRNA 均在外髓晚期近端小管 (S3)、乳头状升细管 (ATL)、外髓 (MTAL) 和皮质 (CTAL) 粗升管、远端小管 (DCT) 以及皮质、外髓和内髓集合管中检测到。间接免疫荧光研究表明，rClC-K 蛋白仅限于 ATL、DCT 和集合管细胞的基底外侧膜，而 CTAL 和 MTAL 表现出更弥散的基底染色。当大鼠脱水时，皮质和髓质中 rClC-K1 的表达增加，在晚期近端小管细胞中发现微弱的细胞质染色。因此，这些结果表明，大鼠肾ClC-K通道主要位于肾小管晚期细胞的基底外侧膜中，大部分氯离子重吸收发生在此处。
We investigate the intrarenal expression of two recently cloned chloride channels, rClC-K1 and rClC-K2, by reverse transcriptase-polymerase chain reaction on single microdissected tubules from the rat kidney and by immunohistochemistry using a polyclonal antibody that recognizes both highly homologous channels. Both rClC-K1 and rClC-K2 mRNAs were detected in outer medullary late proximal tubules (S3), papillary ascending thin limbs (ATL), and outer medullary (MTAL) and cortical (CTAL) thick ascending limbs, distal tubules (DCT), and cortical, outer medullary, and inner medullary collecting ducts. Indirect immunofluorescence studies demonstrated that the rClC-K proteins were restricted to the basolateral membranes from ATL, DCT, and collecting ducts cells, whereas CTAL and MTAL exhibited a more diffuse basal staining. When rats were dehydrated, a condition which increased the expression of rClC-K1 in cortex and medulla, a weak cytoplasmic staining was found in late proximal tubule cells. Thus these results demonstrate that rat kidney ClC-K channels are predominantly located in the basolateral membranes from cells of the late segments of the renal tubule where most of chloride reabsorption takes place.