Neomycin Inhibition of (+)-7-Iso-Jasmonoyl-L-Isoleucine Accumulation and Signaling
Neomycin Inhibition of (+)-7-Iso-Jasmonoyl-L-Isoleucine Accumulation and Signaling
复制标题
新霉素抑制 ( )-7-异-茉莉酰-L-异亮氨酸积累和信号转导
DOI：
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发表时间：
2014
影响因子：
2.3
通讯作者：
A. Mithöfer
中科院分区：
文献类型：
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作者：
J. Vadassery;M. Reichelt;G. Jimenez;W. Boland;A. Mithöfer
大多数植物对食草昆虫的防御是由茉莉酸（茉莉酸，JA;（+）-7-异茉莉酰基-L-异亮氨酸，JA-Ile）依赖的信号级联协调的。昆虫取食和模仿植食动物的应用程序从昆虫的口腔分泌物（OS）诱导细胞质Ca 2+和茉莉酸植物激素的升高。在这里，它表明，在拟南芥处理OS从鳞翅目Spodoptera littoralis幼虫后，抗生素新霉素选择性地阻止积累OS诱导的Ca 2+升高和生物活性的JA-Ile的水平，在JA水平。此外，新霉素处理影响拟南芥中JA-Ile响应基因VSP 2和LOX 2的下游表达。新霉素依赖性JA-Ile水平降低部分是由于CYP 94 B3表达增加和随后JA-Ile转化为12-羟基-JA-Ile。这既不是由于酶结合过程的抑制，也不是由于底物的可用性。因此，阻断Ca 2+升高特异性地控制JA-Ile积累和信号传导，提供了对钙在防御昆虫植食性中的作用的深入了解。
The majority of plant defenses against insect herbivores are coordinated by jasmonate (jasmonic acid, JA; (+)-7-iso-jasmonoyl-L-isoleucine, JA-Ile)-dependent signaling cascades. Insect feeding and mimicking herbivory by application of oral secretions (OS) from the insect induced both cytosolic Ca2+ and jasmonate-phytohormone elevation in plants. Here it is shown that in Arabidopsis thaliana upon treatment with OS from lepidopteran Spodoptera littoralis larvae, the antibiotic neomycin selectively blocked the accumulation of OS-induced Ca2+ elevation and level of the bioactive JA-Ile, in contrast to JA level. Furthermore, neomycin treatment affected the downstream expression of JA-Ile-responsive genes, VSP2 and LOX2, in Arabidopsis. The neomycin-dependent reduced JA-Ile level is partially due to increased CYP94B3 expression and subsequent JA-Ile turn-over to12-hydroxy-JA-Ile. It is neither due to the inhibition of the enzymatic conjugation process nor to substrate availability. Thus, blocking Ca2+ elevation specifically controls JA-Ile accumulation and signaling, offering an insight into role of calcium in defense against insect herbivory.