Snake venomics:: Comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina
Snake venomics:: Comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina
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DOI：
10.1002/pmic.200402024
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发表时间：
2005-11-01
期刊：
影响因子：
3.4
通讯作者：
Calvete, JJ
中科院分区：
文献类型：
--
作者：
Bazaa, A;Marrakchi, N;Calvete, JJ
突尼斯的三个最重要的毒蛇的粗毒液的蛋白质组成进行了分析，通过反相高效液相色谱法，N-末端序列分析，MALDI-TOF质量测定，和ingel胰蛋白酶消化，然后通过PMF和CID-MS/MS的选择的肽离子在四极杆线性IT仪器。我们的研究结果表明，Cerastes cerastes，Cerastes vipera和Macrovipera lebetina的毒液蛋白质组由属于几个蛋白质家族的蛋白质组成。然而，每种毒液显示出不同程度的蛋白质组成的复杂性。这三种蛇毒都有一些共同的蛋白质类，但这些毒素的相对含量在每种蛇中是不同的。在另一个频带上，Cerastes物种和Macrovipera lebetina的毒液各自含有独特的成分。突尼斯蛇毒的比较蛋白质组学分析提供了一个可理解的分泌蛋白质的目录，这可能有助于更深入地了解毒液的生物学效应，也可以作为研究单个毒素的结构-功能相关性的起点。
The protein composition of the crude venoms of the three most important vipers of Tunisia was analyzed by RP-HPLC, N-terminal sequence analysis, MALDI-TOF mass determination, and ingel tryptic digestion followed by PMF and CID-MS/MS of selected peptide ions in a quadrupole-linear IT instrument. Our results show that the venom proteomes of Cerastes cerastes, Cerastes vipera, and Macrovipera lebetina are composed of proteins belonging to a few protein families. However, each venom showed distinct degree of protein composition complexity. The three venoms shared a number of protein classes though the relative occurrence of these toxins was different in each snake species. On the other band, the venoms of the Cerastes species and Macrovipera lebetina each contained unique components. The comparative proteomic analysis of Tunisian snake venoms provides a comprehensible catalogue of secreted proteins, which may contribute to a deeper understanding of the biological effects of the venoms, and may also serve as a starting point for studying structure-function correlations of individual toxins.