Ontogeny of the VIP+ interneuron sensory-motor circuit prior to active whisking
Ontogeny of the VIP+ interneuron sensory-motor circuit prior to active whisking
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DOI：
10.1101/2020.07.01.182238
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发表时间：
2020-07
期刊：
影响因子：
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通讯作者：
C. Vagnoni;Liad J. Baruchin;Filippo Ghezzi;Sara Ratti;Z. Molnár;S. Butt
中科院分区：
文献类型：
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作者：
C. Vagnoni;Liad J. Baruchin;Filippo Ghezzi;Sara Ratti;Z. Molnár;S. Butt
用于感觉运动处理的皮层回路的发展需要柱状突触连接和长程突触连接的协调整合。为了了解这种情况在单个神经元水平上是如何发生的，我们探索了表达血管活性肠肽（VIP）的中间神经元整合到小鼠体感皮层的时间线。我们发现分别在 (L)2 层和 L3 VIP+ 中间神经元上出现的长程前运动和柱状谷氨酸能输入存在差异。与此同时，VIP+中间神经元以由内而外的方式与锥体细胞和直接柱中的中间神经元形成传出连接。细胞自主删除命运决定转录因子 Prox1，可以避免 VIP+ 中间神经元的长距离前部运动输入，但会导致局部连接缺陷。体感回路的这种不平衡导致体内自发和感觉诱发的皮层活动改变。这确定了 VIP+ 中间神经元以及更广泛的中间神经元异质性在新皮质形成回路中的关键作用。
Development of the cortical circuits for sensory-motor processing require the coordinated integration of both columnar and long-range synaptic connections. To understand how this occurs at the level of individual neurons we have explored the timeline over which vasoactive intestinal peptide (VIP)-expressing interneurons integrate into mouse somatosensory cortex. We find a distinction in emergent long-range anterior-motor and columnar glutamatergic inputs onto layer (L)2 and L3 VIP+ interneurons respectively. In parallel, VIP+ interneurons form efferent connections onto both pyramidal cells and interneurons in the immediate column in an inside-out manner. Cell-autonomous deletion of the fate-determinant transcription factor, Prox1, spares long-range anterior-motor inputs onto VIP+ interneurons, but leads to deficits in local connectivity. This imbalance in the somatosensory circuit results in altered spontaneous and sensory-evoked cortical activity in vivo. This identifies a critical role for VIP+ interneurons, and more broadly interneuron heterogeneity, in formative circuits of neocortex.