Synergistic interactions of begomoviruses with Sweet potato chlorotic stunt virus (genus Crinivirus) in sweet potato (Ipomoea batatasL.)
Synergistic interactions of begomoviruses with Sweet potato chlorotic stunt virus (genus Crinivirus) in sweet potato (Ipomoea batatasL.)
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DOI：
10.1111/mpp.12200
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发表时间：
2015-06-01
影响因子：
4.9
通讯作者：
Kreuze, Jan
中科院分区：
文献类型：
--
作者：
Cuellar, Wilmer J.;Galvez, Marco;Kreuze, Jan
采用聚合酶链反应（PCR）对来自拉丁美洲和东非的394份甘薯材料进行了菜豆花叶病毒（Begomovirus）的检测，其中46份为阳性。所有这些都在甘薯中无菌，在Ipomoea setosa中产生叶片卷曲和/或失绿。五个最不同的分离物，基于完整的基因组序列，被用来研究与甘薯褪绿矮化病毒（SPCSV），已知引起协同疾病与其他病毒的相互作用。在所有情况下，合并感染导致菜豆花叶病毒滴度增加和SPCSV滴度降低，尽管菜豆花叶病毒分离株之间的变化程度明显不同。卷叶的症状只开发暂时与隔离StV 1和重合的存在下，最高的菜豆花叶病毒浓度的植物。小干扰RNA（siRNA）序列分析表明，SPCSV与分离StV 1的共感染导致相对增加的siRNA靶向的SPCSV基因组的中心部分和减少靶向的基因组末端，但没有变化的靶向StV 1相对于单一感染的病毒。在SPCSV和RNA病毒甘薯羽状斑驳病毒（马铃薯Y病毒属）之间的相互作用中没有观察到这些变化，这意味着双生子病毒对双重感染植物中SPCSV的RNA沉默的特异性影响。在RNase 3表达转基因植物的感染表明，这种蛋白质是足以介导这种协同作用与DNA病毒，类似于RNA病毒，但暴露不同的影响RNA沉默时，RNase 3从其天然病毒表达，或组成型从转基因，尽管类似的致病性结果。
Three hundred and ninety-four sweet potato accessions from Latin America and East Africa were screened by polymerase chain reaction (PCR) for the presence of begomoviruses, and 46 were found to be positive. All were symptomless in sweet potato and generated leaf curling and/or chlorosis in Ipomoea setosa. The five most divergent isolates, based on complete genome sequences, were used to study interactions with Sweet potato chlorotic stunt virus (SPCSV), known to cause synergistic diseases with other viruses. Co-infections led to increased titres of begomoviruses and decreased titres of SPCSV in all cases, although the extent of the changes varied notably between begomovirus isolates. Symptoms of leaf curling only developed temporarily in combination with isolate StV1 and coincided with the presence of the highest begomovirus concentrations in the plant. Small interfering RNA (siRNA) sequence analysis revealed that co-infection of SPCSV with isolate StV1 led to relatively increased siRNA targeting of the central part of the SPCSV genome and a reduction in targeting of the genomic ends, but no changes to the targeting of StV1 relative to single infection of either virus. These changes were not observed in the interaction between SPCSV and the RNA virus Sweet potato feathery mottle virus (genus Potyvirus), implying specific effects of begomoviruses on RNA silencing of SPCSV in dually infected plants. Infection in RNase3-expressing transgenic plants showed that this protein was sufficient to mediate this synergistic interaction with DNA viruses, similar to RNA viruses, but exposed distinct effects on RNA silencing when RNase3 was expressed from its native virus, or constitutively from a transgene, despite a similar pathogenic outcome.