Polypyrrole DNA chip on a silicon device:: Example of hepatitis C virus genotyping
Polypyrrole DNA chip on a silicon device:: Example of hepatitis C virus genotyping
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DOI：
10.1006/abio.1997.2462
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发表时间：
1998-01-15
影响因子：
2.9
通讯作者：
Mathis, G
中科院分区：
文献类型：
--
作者：
Livache, T;Fouque, B;Mathis, G
在这篇文章中，我们描述了一个寡核苷酸阵列上的硅设备轴承矩阵的可寻址的50 μ m微电极构建。每个电极都被一种接枝有寡核苷酸（ODN）的导电聚合物（聚吡咯）覆盖。DNA芯片是通过5'吡咯标记的ODN与吡咯的连续电化学寻址聚合制备的。在生物素化的扩增样品在带有一系列探针的芯片上杂交后，通过荧光显微镜通过R-藻红蛋白标记进行检测。该技术已成功应用于血液样本中丙型肝炎病毒的基因分型。结果表明，良好的灵敏度和高度的空间分辨率。(C)北京：科学出版社.
We describe in this article an oligonucleotide array constructed on a silicon device bearing a matrix of addressable 50-mu m microelectrodes. Each electrode was covered by a conducting polymer (polypyrrole) grafted by an oligonucleotide (ODN). The DNA chip was prepared by successive electrochemically addressed copolymerizations of 5' pyrrole-labeled ODN and pyrrole. Following hybridization of the biotinylated amplified sample on the chip bearing a series of probes, detection was carried out by fluorescence microscopy through an R-phycoerythrin label. This technology was successfully applied to the genotyping of hepatitis C virus in blood samples. Results show good sensitivity and a high degree of dimensional resolution. (C) 1998 Academic Press.