Whole-genome analysis of Oryza sativa reveals similar architecture of two-component signaling machinery with arabidopsis
Whole-genome analysis of Oryza sativa reveals similar architecture of two-component signaling machinery with arabidopsis
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DOI：
10.1104/pp.106.086371
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发表时间：
2006-10-01
期刊：
影响因子：
7.4
通讯作者：
Singla-Pareek, Sneh L.
中科院分区：
文献类型：
--
作者：
Pareek, Ashwani;Singh, Anupama;Singla-Pareek, Sneh L.
双组分系统（TCS）是一种以组氨酸-天冬氨酸磷酸化传递信号为基础的信号系统，在低等生物的多种生理过程中发挥着重要作用，近年来已成为植物中一种重要的信号系统。利用生物信息学的工具，我们对水稻基因组中TCS信号候选基因进行了分析。亚种日本的。本文对O.包括基因结构、保守基序、染色体位置和染色体发生。我们的分析表明，共有51个基因编码73个推定的TCS蛋白。14个基因编码22个假定的组氨酸激酶，具有保守的组氨酸和其他典型的组氨酸激酶特征序列，5个磷酸转移基因编码7个磷酸转移蛋白，32个反应调节基因编码44种蛋白。基因和蛋白质数量之间的变化被认为是可变剪接的结果。这些推定的蛋白质与TCS成员具有高度同源性，TCS成员已被实验证明参与植物中的几个重要生理现象，如乙烯和细胞分裂素信号传导以及光敏色素介导的对光的响应。我们的结论是，整体架构的TCS机制在O。sativa和拟南芥是相似的，我们的分析提供了深入了解高等植物中这一重要信号机制的保守性和分歧。
The two-component system (TCS), which works on the principle of histidine-aspartate phosphorelay signaling, is known to play an important role in diverse physiological processes in lower organisms and has recently emerged as an important signaling system in plants. Employing the tools of bioinformatics, we have characterized TCS signaling candidate genes in the genome of Oryza sativa L. subsp. japonica. We present a complete overview of TCS gene families in O. sativa, including gene structures, conserved motifs, chromosome locations, and phylogeny. Our analysis indicates a total of 51 genes encoding 73 putative TCS proteins. Fourteen genes encode 22 putative histidine kinases with a conserved histidine and other typical histidine kinase signature sequences, five phosphotransfer genes encoding seven phosphotransfer proteins, and 32 response regulator genes encoding 44 proteins. The variations seen between gene and protein numbers are assumed to result from alternative splicing. These putative proteins have high homology with TCS members that have been shown experimentally to participate in several important physiological phenomena in plants, such as ethylene and cytokinin signaling and phytochrome-mediated responses to light. We conclude that the overall architecture of the TCS machinery in O. sativa and Arabidopsis thaliana is similar, and our analysis provides insights into the conservation and divergence of this important signaling machinery in higher plants.