Calcium Phosphate Mineralized Black Phosphorous with Enhanced Functionality and Anticancer Bioactivity
Calcium Phosphate Mineralized Black Phosphorous with Enhanced Functionality and Anticancer Bioactivity
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具有增强功能和抗癌生物活性的磷酸钙矿化黑磷
DOI：
10.1002/adfm.202003069
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发表时间：
2020-07-16
影响因子：
19
通讯作者：
Yu, Xue-Feng
中科院分区：
文献类型：
--
作者：
Pan, Ting;Fu, Wen;Yu, Xue-Feng
可生物降解的无机纳米材料由于其固有的抗癌活性，为癌症治疗开辟了新的前景。黑磷纳米片（BPs）具有独特的生物活性，近年来被认为是一种很有前途的肿瘤治疗剂，但其表面功能化的困难阻碍了其应用。在此，原位磷酸钙（CaP）矿化策略被描述为增强BPs的抗癌活性。通过使用BPs作为磷酸盐来源和生长模板，合成的CaP矿化BPs（CaBPs）保留了BPs的固有特性，同时具有对各种荧光团的高负载能力，以实现有效的生物成像和追踪。与BPs相比，CaBPs具有更强的选择性抗癌活性，这是由于CaBPs具有更好的pH响应性降解行为和细胞内Ca（2+）超载。此外，CaBP特异性靶向线粒体并引起结构损伤，从而导致癌细胞中的细胞凋亡。静脉注射后，CaBP靶向原位乳腺癌细胞以抑制肿瘤生长，而不会产生副作用或毒性。结果表明CaBP作为靶向抗癌剂的巨大潜力，CaP矿化方法为纳米药物提供了一种通用的表面功能化策略。
Biodegradable inorganic nanomaterials have opened new perspectives for cancer therapy due to their inherent anticancer activity. Black phosphorus nanosheets (BPs) with their unique bioactivity have recently been identified as promising cancer therapeutic agents but their application is hampered by the difficulty in surface functionalization. Herein, an in situ calcium phosphate (CaP) mineralization strategy is described to enhance the anticancer activity of BPs. By using BPs as the phosphate sources and growth templates, the synthesized CaP-mineralized BPs (CaBPs) retain the intrinsic properties of BPs and at the same time have high loading capacities for various fluorophores to enable effective bioimaging and tracing. Compared to BPs, CaBPs exhibit enhanced and selective anticancer bioactivity due to the improved pH-responsive degradation behavior and intracellular Ca(2+)overloading in cancer cells. Furthermore, CaBPs specifically target mitochondria and cause structural damage, thus leading to mitochondria-mediated apoptosis in cancer cells. After intravenous injection, CaBPs target orthotopic breast cancer cells to inhibit tumor growth without giving rise to adverse effects or toxicity. The results demonstrate the great potential of CaBPs as targeted anticancer agents and the CaP mineralization approach provides a versatile surface functionalization strategy for nanotherapeutic agents.