The effect of tides on nearshore environmental DNA
The effect of tides on nearshore environmental DNA
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DOI：
10.7717/peerj.4521
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发表时间：
2018-03-19
期刊：
影响因子：
2.7
通讯作者：
Jacobs-Palmer, Emily
中科院分区：
文献类型：
--
作者：
Kelly, Ryan P.;Gallego, Ramon;Jacobs-Palmer, Emily
我们可以通过环境采样从各种生物体中恢复遗传信息。近年来，对这种环境DNA（eDNA）进行测序已成为使用水，空气或土壤样本调查许多物种的一种易于处理的方法。该技术开始成为生态学家，环境科学家和生物学家的核心工具，但eDNA采样的时间分辨率通常不清楚，限制了对所得数据集的生态解释。在这里，在一个时间和空间复制领域的研究，使用ca。313 bp的真核生物COI mtDNA作为标记，我们发现，近岸生物群落在很大程度上是一致的跨潮汐。我们的研究结果表明，近岸eDNA从底栖和浮游生物类群往往是内源性的网站和水质量采样，而不是随着每个潮汐周期的变化。然而，在物理化学的水团特征的变化，我们发现，广泛的生物eDNA社区的相对贡献一致的转变。
We can recover genetic information from organisms of all kinds using environmental sampling. In recent years, sequencing this environmental DNA (eDNA) has become a tractable means of surveying many species using water, air, or soil samples. The technique is beginning to become a core tool for ecologists, environmental scientists, and biologists of many kinds, but the temporal resolution of eDNA sampling is often unclear, limiting the ecological interpretations of the resulting datasets. Here, in a temporally and spatially replicated field study using ca. 313 bp of eukaryotic COI mtDNA as a marker, we find that nearshore organismal communities are largely consistent across tides. Our findings suggest that nearshore eDNA from both benthic and planktonic taxa tends to be endogenous to the site and water mass sampled, rather than changing with each tidal cycle. However, where physiochemical water mass characteristics change, we find that the relative contributions of a broad range of organisms to eDNA communities shift in concert.