Process optimization for acidic deep eutectic solvent pretreatment of corn stover to enhance enzymatic saccharification
Process optimization for acidic deep eutectic solvent pretreatment of corn stover to enhance enzymatic saccharification
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DOI：
10.1007/s13399-022-02738-0
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发表时间：
2022-05
影响因子：
4
通讯作者：
Tianjie Ao;Kai Li;M. Mehmood;Xinqing Zhao;F. Bai;R. Boopathy;Chen-Guang Liu
中科院分区：
文献类型：
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作者：
Tianjie Ao;Kai Li;M. Mehmood;Xinqing Zhao;F. Bai;R. Boopathy;Chen-Guang Liu
本研究采用深度共晶溶剂（DES）对顽固性玉米秸秆（CS）进行有效预处理，以提高后续糖化工艺的效率。通过响应面法研究乳酸(LA)/氯化胆碱（ChCl）的摩尔比、温度和时间，优化预处理条件。当LA/ChCl摩尔比为1时，纤维素和半纤维素的消化率（94.85%）和脱木质素效率（29.27%）最高，温度为140°C，处理4 h。总体而言，与未经处理的CS相比，这一效率提高了近7倍。此外，在较高的温度下（约140℃），预处理过程中产生了人蛋白，这些人蛋白可能包裹在预处理后的CS表面，导致酶的可及性受阻。经过3次重复循环后，预处理效果也完全保持。该研究为LA/ChCl型DES在木质纤维素生物质预处理中的综合利用提供了深入的见解。图形抽象
In the present study, deep eutectic solvent (DES) was employed for an effective pretreatment of recalcitrant corn stover (CS) to improve the efficiency of the subsequent saccharification process. Accordingly, the molar ratios of lactic acid (LA)/choline chloride (ChCl), temperature, and time were studied via response surface methodology to optimize pretreatment conditions. While the best cellulose and hemicelluloses digestibility (94.85%) and delignification (29.27%) efficiency were achieved using the LA/ChCl molar ratio of 1 at 140 °C after 4 h. Overall, this efficiency was nearly seven-fold higher when compared to the untreated CS. Besides, at higher temperatures (> 140 °C), the humins were generated in the pretreatment process, which might have wrapped around the surface of the pretreated CS and resulted in an impeded enzymatic accessibility. The pretreatment efficiency was also fully maintained after three repeated cycles. This study provides an insight into the comprehensive utilization of the LA/ChCl type of DES for robust lignocellulosic biomass pretreatment.Graphical abstract