Identification of the secreted watery saliva proteins of the rice brown planthopper, Nilaparvata lugens (Stal) by transcriptome and Shotgun LC-MS/MS approach
Identification of the secreted watery saliva proteins of the rice brown planthopper, Nilaparvata lugens (Stal) by transcriptome and Shotgun LC-MS/MS approach
复制标题
通过转录组和鸟枪法 LC-MS/MS 方法鉴定稻褐飞虱 Nilaparvata lugens (Stal) 分泌的水状唾液蛋白
DOI：
10.1016/j.jinsphys.2016.04.002
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发表时间：
2016-06-01
影响因子：
2.2
通讯作者：
He, Yueping
中科院分区：
文献类型：
--
作者：
Liu, Xiaoqing;Zhou, Hanyu;He, Yueping
水稻褐飞虱是亚洲水稻的主要害虫，是一种维管取食昆虫，在取食过程中产生胶凝和水状唾液。虽然N.尽管已经使用2D PAGE鉴定了褐飞虱，但是对这种昆虫分泌的唾液知之甚少。在这项研究中，我们确定了主要蛋白质分泌的水样唾液中的N。通过拉伸的石蜡膜从稻飞虱成虫取食的蔗糖饲料中收集样品，并参考N. lugens的唾液腺转录组数据库，使用鸟枪LC-MS/MS分析。lugens。结果表明，在中华绒螯蟹水样唾液中共鉴定出107种蛋白质。褐飞虱，其中超过80%与已知蛋白质具有显着相似性。当用Blast 2GO套件注释时，29个蛋白质具有催化活性，24个蛋白质是结合蛋白。唾液酶包括氧化还原酶、水解酶、磷酸酶、肽酶（蛋白酶）、激酶、转移酶和裂解酶。N.褐变水样唾液包括ATP结合蛋白、载脂蛋白、钙结合蛋白、肌动蛋白结合蛋白以及DNA、RNA和染色质结合蛋白。其他非酶蛋白如泛素、热休克蛋白、核糖体蛋白和免疫球蛋白等也在N.褐色水样唾液。这是首次对中华白蛉水样唾液中的蛋白质进行鉴定、表征和分类的研究。lugens可能参与稻飞虱-水稻互作。(C)2016爱思唯尔有限公司版权所有
The rice brown planthopper, Nilaparvata lugens (Stal), a major rice insect pest in Asia, is a vascular bundle-feeder that ejects gelling and watery saliva during the feeding process. Although major proteins in the salivary glands of N. lugens have been identified using 2D PAGE, very little is known about the secreted saliva of this insect. In this study, we identified the major proteins in the secreted watery saliva of N. lugens, via collecting from a sucrose diet that adult planthoppers had fed upon through a membrane of stretched parafilm, and using shotgun LC-MS/MS analysis with reference to transcriptome database of salivary glands of N. lugens. A total of 107 proteins were identified in the watery saliva of N. lugens, over 80% of which showed significant similarity to known proteins. When annotated by the Blast2GO suite, 29 proteins had catalytic activity and 24 proteins were binding proteins. The saliva enzymes included oxidoreductases, hydrolases, phosphatases, peptidases (proteases), kinases, transferases, and lyases. Binding proteins in N. lugens watery saliva included ATP-binding, lipophorin, calcium-binding, actin-binding and DNA-, RNA-, and chromatin-binding proteins. Other non-enzymatic proteins, such as ubiquitins, heat shock proteins, ribosomal proteins, and immunoglobulin proteins were also found in N. lugens watery saliva. This is the first study to identify, characterize and list the proteins in watery saliva of N. lugens, which might be involved in planthopper-rice interactions. (C) 2016 Elsevier Ltd. All rights reserved.