Identification of a LPS-induced TNF-α factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation.
Identification of a LPS-induced TNF-α factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation.
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DOI：
10.1016/j.fsi.2013.07.034
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发表时间：
2013-10
影响因子：
4.7
通讯作者：
Dinglong Yang;Xiumei Wei;Jianmin Yang;Jialong Yang;Jie Xu;Jinghui Fang;Sheng Wang;Xiangquan Liu
中科院分区：
文献类型：
--
作者：
Dinglong Yang;Xiumei Wei;Jianmin Yang;Jialong Yang;Jie Xu;Jinghui Fang;Sheng Wang;Xiangquan Liu
脂多糖诱导的肿瘤坏死因子-α是介导肿瘤坏死因子-α转录的重要转录因子之一。在本研究中，从沙棘中鉴定了一个LITAF基因(命名为SgLITAF)。SgLITAF全长为1476hp，编码130个氨基酸的多肽，与已知的LITAFs有很高的同源性。SgLITAF编码一个LITAF结构域，结构域中的锌结合基序非常保守。SgLITAF基因在健康文蛤的外套膜、鳃、性腺、血细胞、肌肉和肝胰腺等组织中均有表达，其中以肝胰腺的表达水平最高。细菌脂多糖或β-1，3-葡聚糖刺激后，血细胞中SgLITAF的表达水平显著上调(P<；P<0.01)，而PGN刺激后SgLITAF的表达水平无明显变化。以上结果表明，巨噬细胞中可能存在LITAF调节的肿瘤坏死因子-α信号通路，该信号通路不仅参与了对革兰氏阴性菌的免疫应答，也参与了对真菌的免疫应答。
Lipopolysaccharide-induced TNF-α factor (LITAF) is one of the most important transcription factors mediating TNF-α transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clamsSolen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P< 0.01) after razor clams were stimulated by LPS or β-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-α signaling pathway existing inS.grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi.