Preparations of duck hepatitis B virions contain multiple DNA polymerase activities.
Preparations of duck hepatitis B virions contain multiple DNA polymerase activities.
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鸭乙型肝炎病毒颗粒制剂含有多种 DNA 聚合酶活性。
DOI：
10.1006/viro.1996.0636
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Newbold,JE
中科院分区：
文献类型：
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作者：
Oberhaus,SM;Newbold,JE
嗜肝DNA病毒基因组由病毒编码的逆转录酶合成，但这种蛋白质在体内的性质仍然不清楚。我们以前描述的研究中，活性凝胶法确定了多种DNA聚合酶（DNAp）的活动与高度纯化的鸭B肝炎病毒（DHBV）的核心颗粒。我们现在报告，从病毒血症血清中分离的病毒粒子与DNA依赖的DNAp活动，这是几乎相同的主要DNAp活动与高度纯化的DHBV核心颗粒检测。这些结果表明，病毒粒子相关的聚合酶是相同的，与核心颗粒检测，并可能代表参与基因组复制的DHBVpolgene产品。
The hepadnaviral DNA genome is synthesized by a viral-encoded reverse transcriptase, but the nature of this protein(s)in vivoremains obscure. We have previously described studies in which activity gel assays identified multiple DNA polymerase (DNAp) activities associated with highly purified duck hepatitis B virus (DHBV) core particles. We now report that virions isolated from viremic sera are associated with DNA-dependent DNAp activities which are nearly identical to major DNAp activities detected with highly purified DHBV core particles. These results suggest that the virion-associated polymerases are the same as those which are detected with core particles and are likely to represent DHBVpolgene products involved in replication of the genome.