Nuclear localization of unoccupied receptors for glucocorticoids, estrogens, and progesterone in GH3 cells.
Nuclear localization of unoccupied receptors for glucocorticoids, estrogens, and progesterone in GH3 cells.
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DOI：
10.1210/endo-117-5-2140
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发表时间：
1985-11
期刊：
影响因子：
4.8
通讯作者：
W. Welshons;B. Krummel;J. Gorski
中科院分区：
文献类型：
--
作者：
W. Welshons;B. Krummel;J. Gorski
我们之前已经表明，雌激素的未占用受体不存在于去核细胞（细胞质）中，并且在使用细胞松弛素B和离心去核的细胞中可能是核的。我们现在证明，在不使用细胞松弛素的情况下去核，糖皮质激素和黄体酮的未被占用的受体似乎也是有核的，不使用细胞松弛素的去核导致雌激素受体的分布与使用这种药物时相同。去核细胞（细胞质）中未被占用受体的浓度仅为整个细胞中未被占用受体的5-10%。所有三种激素的未占用受体在含核细胞片段（核质体）中被恢复。细胞质中乳酸脱氢酶的浓度与整个细胞或核质体相同或更高。细胞质由大约90%的细胞形成。当核体匀浆后，三种激素的未占用受体被提取到细胞质中，这在匀浆后的细胞和组织中是常见的。因此，这些结果与未被占用的糖皮质激素和孕激素受体，像未被占用的雌激素受体一样，是核蛋白而不是细胞质蛋白的可能性是一致的。此外，这些未填充受体的定位似乎不是细胞松弛素治疗的产物。
We have previously shown that the unoccupied receptor for estrogen is not present in enucleated cells (cytoplasts) and probably is nuclear in cells that have been enucleated using cytochalasin B and centrifugation. We now demonstrate, using enucleation without cytochalasin, that the unoccupied receptors for glucocorticoids and progesterone also appear to be nuclear, and that enucleation without using cytochalasin results in the same distribution of estrogen receptor as that seen when this drug is used. Enucleated cells (cytoplasts) contained only 5-10% of the concentration of unoccupied receptors found in the whole cells. The unoccupied receptors for all three hormones were recovered instead in the nucleus-containing cell fragments (nucleoplasts). The cytosolic marker lactate dehydrogenase was present in the cytoplasts at the same or higher concentration than in the whole cells or nucleoplasts. Cytoplasts were formed from approximately 90% of the cells. When the nucleoplasts were homogenized, the unoccupied receptors for all three hormones were extracted into the cytosol, as is usually seen in homogenized cells and tissues. These results are, therefore, consistent with the possibility that the unoccupied glucocorticoid and progesterone receptors, like the unoccupied estrogen receptor, are nuclear rather than cytoplasmic proteins. In addition, localization of these unfilled receptors does not appear to be an artifact of cytochalasin treatment.