Cytological and transcriptome analysis reveal that interaction at Sb pollen sterility locus cause down-regulation of important meiosis-related genes associated with high pollen sterility in autotetraploid rice hybrids
Cytological and transcriptome analysis reveal that interaction at Sb pollen sterility locus cause down-regulation of important meiosis-related genes associated with high pollen sterility in autotetraploid rice hybrids
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细胞学和转录组分析表明，Sb 花粉不育位点的相互作用导致与同源四倍体水稻杂交种高花粉不育相关的重要减数分裂相关基因下调
DOI：
10.1016/j.plaphy.2019.05.019
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发表时间：
2019
影响因子：
6.5
通讯作者：
Liu Xiangdong
中科院分区：
文献类型：
--
作者：
Wu Jinwen;Shahid Muhammad Qasim;Chen Minyi;Li Xiang;Li Jirui;Xu Xiaosong;Du Susu;Liu Xiangdong
多倍体可以增加花粉不育位点的相互作用，并且与其他位点相比，Sblocus相互作用导致更高的花粉败育率。因此，我们在本研究中使用近等基因系，重点关注同源四倍体水稻与二倍体水稻杂交体中Sb花粉不育位点的相互作用。细胞学观察表明，Sblocus 的相互作用导致同源四倍体水稻杂交种中期 II 的高花粉不育性 (69.9%) 和异常染色体行为 (37.02%)。在具有相互作用的同源四倍体水稻杂交种Sblocus中总共检测到139个减数分裂相关或减数分裂阶段特异性基因，其中27个减数分裂相关或特定基因表现出显着下调，其中包括4个花粉育性相关基因（Rad51、XRI1、PSS1和MIL1）。这些结果表明，Sb花粉不育位点的相互作用比其他位点更强，这导致许多重要的减数分裂相关基因的下调，这些基因与同源四倍体水稻杂交种中较高的花粉不育性相关。
Polyploidy could increase the interactions of pollen sterility loci andSblocus interaction cause higher pollen abortion than other loci. Therefore, we focused on the interaction atSbpollen sterility locus in autotetraploid rice compared to diploid rice hybrid using the near-isogenic lines in the present study. Cytological observations indicated that interaction atSblocus cause high pollen sterility (69.9%) and abnormal chromosome behavior (37.02%) at Metaphase II in autotetraploid rice hybrid. A total of 139 meiosis-related or meiosis stage-specific genes were detected in the autotetraploid rice hybrid harboring interaction atSblocus and 27 of these meiosis-related or specific genes displayed significant down-regulation, including four pollen fertility related genes (Rad51,XRI1,PSS1andMIL1). These results revealed a stronger interaction atSbpollen sterility locus than other loci, which cause down-regulation of many important meiosis-related genes that were associated with higher pollen sterility in autotetraploid rice hybrids.