Rates of electron transport in the thylakoid membranes of isolated, illuminated chloroplasts are enhanced in the presence of ammonium chloride
Rates of electron transport in the thylakoid membranes of isolated, illuminated chloroplasts are enhanced in the presence of ammonium chloride
复制标题
DOI：
10.1002/bmb.2003.494031060265
复制
发表时间：
2003-11-01
影响因子：
1.4
通讯作者：
Miskiewicz, E
中科院分区：
文献类型：
--
作者：
Dean, RL;Miskiewicz, E
电子从水到人工电子受体，二氯酚靛酚（DCPIP），通过光系统II中的类囊体膜分离的叶绿体的流量大大提高在10 mM氯化铵的存在下。速率增强取决于辐照度水平。解偶联试剂如氯化铵防止跨类囊体膜形成质子梯度，从而消除对电子传递速率的限制。说明了氯化铵的作用方式。使用氧电极获得的证据表明，DCPIP本身也部分解耦的系统作为背景信息的教师。如何这种反应可能会被用于从高中到高级本科生水平的学生在实验室类的建议。
The rate of flow of electrons from water to an artificial electron acceptor, dichlorophenolindophenol (DCPIP), through photosystem II in the thylakoid membranes of isolated chloroplasts is greatly enhanced in the presence of 10 mM ammonium chloride. Rate enhancement depends on irradiance levels. Uncoupling reagents like ammonium chloride prevent the formation of a proton gradient across the thylakoid membrane and consequently remove a constraint on the rate of electron transport. The mode of action of ammonium chloride is explained. Evidence obtained using an oxygen electrode that DCPIP itself also partially uncouples the system is presented as background information for instructors. Suggestions on how this reaction may be used in laboratory classes for students from high school to the senior undergraduate level are included.