Characterization of a novel protein identified by proteomics analysis as a modulator of inflammatory networks in amphioxus
Characterization of a novel protein identified by proteomics analysis as a modulator of inflammatory networks in amphioxus
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通过蛋白质组学分析鉴定为文昌鱼炎症网络调节剂的新型蛋白质的表征
DOI：
10.1016/j.fsi.2019.12.003
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发表时间：
2019
影响因子：
4.7
通讯作者：
Shicui Zhang
中科院分区：
文献类型：
--
作者：
Baozhen Qu;Zengyu Ma;Yu Zhang;Zhan Gao;Shicui Zhang
炎症反应是宿主的一种先天防御机制，其调控是宿主摆脱过度反应伤害的必要条件。我们首先利用蛋白质组学方法鉴定文昌鱼体液蛋白对lps诱导炎症的反应。在lps处理动物和对照动物之间，共鉴定出26种差异表达蛋白，主要参与能量代谢和细胞骨架重排过程。此外，我们在上调最多的蛋白中发现了一个未表征的蛋白（称为BjIM1），并研究了其在调节免疫和炎症反应中的作用。bjim1主要表达于肝盲肠，其启动子序列包含许多免疫相关转录因子的结合位点。重要的是，重组BjIM1 （rBjIM1）能够抑制lps诱导的myd88、IKK、NF-κB1、Rel、p38、JNKandAP-1等TLR通路基因的上调，表明BjIM1可能负向调控文文鱼的TLR信号通路。此外，rBjIM1还能调节文文鱼脂多糖诱导炎症反应中参与炎症、能量代谢和细胞骨架重排相互作用网络的基因sirt1、rac1和nox2的表达。总的来说，我们的研究表明BjIM1是文昌鱼炎症网络调节剂的一种未被描述的蛋白质。
Inflammatory response is an innate host defense mechanism, and its regulation is essential for the host to get rid of harm by the excessive reactions. We first utilized proteomics approach to identify amphioxus humoral fluid proteins in response to LPS-induced inflammation. A total of 26 differentially expressed proteins, mainly involved in energy metabolism and cytoskeleton rearrangement processes, were identified between LPS-treated and control animals. Furthermore, we found a single uncharacterized protein (termed BjIM1) out of the most up-regulated ones, and examined its role in the regulation of immune and inflammatory responses.BjIM1is predominantly expressed in the hepatic caecum, and its promoter sequence includes many binding sites for immune-relevant transcription factors. Importantly, recombinant BjIM1 (rBjIM1) is able to inhibit LPS-induced up-regulation of TLR pathway genes, such asMyD88,IKK,NF-κB1,Rel,p38,JNKandAP-1, indicating that BjIM1 may negatively regulate the TLR signaling pathway in amphioxus. Moreover, rBjIM1 also modulates the expression of genes involved in the interaction network of inflammation, energy metabolism and cytoskeleton rearrangement, includingSIRT1,Rac1andNOX2, in the LPS-induced inflammatory response in amphioxus. Collectively, our studies suggest that BjIM1 is an uncharacterized protein functioning as a modulator of inflammatory networks in amphioxus.