Differentiation of female primordial germ cells in the male testes of chicken (Gallus gallus domesticus)
Differentiation of female primordial germ cells in the male testes of chicken (Gallus gallus domesticus)
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DOI：
10.1002/mrd.20499
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发表时间：
2007-01-01
影响因子：
2.5
通讯作者：
Nirasawa, Keijiro
中科院分区：
文献类型：
--
作者：
Tagami, Takahiro;Kagami, Hiroshi;Nirasawa, Keijiro
在我们以前的研究中，我们证明了雌性原始生殖细胞（PGCs）在生殖系嵌合体鸡的雄性性腺中具有分化为带有W染色体（W-轴承）的精子的能力。本研究通过将雌性PGCs注射到雄性受体鸡胚中，构建了3只生殖系嵌合体鸡，以探讨雌性PGCs在雄性生殖腺中的分化规律。在这些雄性嵌合体达到性成熟后，通过PCR和原位杂交分析对精液样品进行分析以检测含W细胞。结果表明，雌性原始生殖细胞已在精巢内定居并分化。对这些嵌合体的曲细精管进行组织学分析表明，含W的精原细胞、精母细胞和圆形精子细胞分别占30.8%、32.7%和28.4%。然而，含W的伸长精子细胞（7.7%）明显低于含W的圆形精子细胞。几乎没有观察到含W精子（0.2%）。我们的结论是，虽然男性性腺中的女性PGCs能够通过第一次和第二次减数分裂，以适应男性的环境，他们很难完成精子发生。
In our previous studies, we demonstrated that female primordial germ cells (PGCs) have the ability to differentiate into W chromosome-bearing (W-bearing) spermatozoa in male gonads of germline chimeric chickens. In this study, to investigate the differentiation pattern of female PGCs in male gonads in chickens, three germline chimeric chickens were generated by injecting female PGCs into the male recipient embryos. After these male chimeras reached sexual maturity, the semen samples were analyzed for detecting W-bearing cells by PCR and in situ hybridization analyses. The results indicated that the female PGCs had settled and differentiated in their testes. A histological analysis of the seminiferous tubule in those chimeras demonstrated that the W-bearing spermatogonia, spermatocytes, and round spermatids accounted for 30.8%, 32.7%, and 28.4%, respectively. However, the W-bearing elongating spermatid was markedly lower (7.7%) as compared to the W-bearing round spermatid. The W-bearing spermatozoa were hardly ever observed (0.2%). We concluded that although female PGCs in male gonads are capable of passing through the first and second meiotic division in adapting themselves to a male environment, they are hardly complete spermiogenesis.