MONOAMINERGIC CONTROL OF THE RELEASE OF CALCITONIN-GENE-RELATED PEPTIDE-LIKE AND SUBSTANCE P-LIKE MATERIALS FROM RAT SPINAL-CORD SLICES
MONOAMINERGIC CONTROL OF THE RELEASE OF CALCITONIN-GENE-RELATED PEPTIDE-LIKE AND SUBSTANCE P-LIKE MATERIALS FROM RAT SPINAL-CORD SLICES
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DOI：
10.1016/0028-3908(93)90076-f
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发表时间：
1993-07-01
影响因子：
4.7
通讯作者：
CESSELIN, F
中科院分区：
文献类型：
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作者：
BOURGOIN, S;POHL, M;CESSELIN, F
可能的控制由单胺类物质的P-和降钙素基因相关肽样物质（SPLM和CGRPLM，分别）的脊髓释放进行了研究，在体外，使用切片的背侧一半的大鼠腰椎扩大灌注人工脑脊液。而自发流出的SPLM和CGRPLM没有改变测试的单胺受体的激动剂/拮抗剂，溢出的两个肽样物质由于30 mM K+的差异影响α 2-肾上腺素受体和多巴胺D-1受体配体。去甲肾上腺素（10 μ M至0.1 mM）和可乐定（0.1 mM）显着减少了K+诱发的SPLM溢出，这两种作用可以防止由idazoxan（10 μ M）和哌唑嗪（10 μ M），因为它们通过刺激α 2B-肾上腺素受体介导的预期。相反，CGRPLM溢出保持不受α 2-肾上腺素受体配体的影响。SKF 82958（10-100 nM）对多巴胺D-1受体的刺激显著增加了SPLM和CGRPLM的K+诱发溢出，而选择性D-1拮抗剂SCH 39166（1 μ M）可阻止这种效应。对其他单胺受体的选择性配体的进一步研究表明，α 1-和β-肾上腺素能受体、多巴胺D-2、5-羟色胺5-HT 1A和5-HT 3受体都不明显参与对CGRPLM和SPLM的脊髓释放的某些控制。这些数据进行了讨论，与假定的突触前控制单胺的初级传入纤维内脊髓背角传递伤害性信息。
The possible control by monoamines of the spinal release of substance P- and calcitonin gene-related peptide-like materials (SPLM and CGRPLM, respectively) was investigated in vitro, using slices of the dorsal half of the rat lumbar enlargement superfused with an artificial cerebrospinal fluid. Whereas the spontaneous outflow of SPLM and CGRPLM was changed by none of the agonists/antagonists of monoamine receptors tested, the overflow of both peptide-like materials due to 30 mM K+ was differentially affected by alpha2-adrenoreceptor and dopamine D-1 receptor ligands. Noradrenaline (10 muM to 0.1 mM) and clonidine (0.1 mM) significantly reduced the K+-evoked overflow of SPLM, and both effects could be prevented by idazoxan (10 muM) and prazosin (10 muM) as expected from their mediation through the stimulation of alpha2B-adrenoreceptors. In contrast, CGRPLM overflow remained unaffected by alpha2-adrenoreceptor ligands. Dopamine D-1 receptor stimulation by SKF 82958 (10-100 nM) significantly increased the K+-evoked overflow of both SPLM and CGRPLM, and this effect could be prevented by the selective D-1 antagonist SCH 39166 (1 muM). Further studies with selective ligands of other monoamine receptors indicated that neither alpha1- and beta-adrenergic receptors, dopamine D-2, nor serotonin 5-HT1A and 5-HT3 receptors are apparently involved in some control of the spinal release of CGRPLM and SPLM. These data are discussed in line with the postulated presynaptic control by monoamines of primary afferent fibres conveying nociceptive messages within the dorsal horn of the spinal cord.