Evaluation of the anticancer activity of Origanum Marjoram as a safe natural drink for daily use
Evaluation of the anticancer activity of Origanum Marjoram as a safe natural drink for daily use
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DOI：
10.1080/03639045.2023.2257796
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发表时间：
2023-09-12
影响因子：
3.4
通讯作者：
Alsharidah, Mansour
中科院分区：
文献类型：
--
作者：
Abdellatif, Ahmed A. H.;Alsharidah, Mansour
背景：化疗药物有许多副作用。人们对使用天然和安全的植物作为食物或饮料来预防癌症很感兴趣。马乔兰（Origanum marjoram， OMAE）是一种药用植物，可作为茶叶、食品和传统医学添加剂使用。目的：本研究旨在评价OMAE作为日常饮用软饮料对模型癌的潜在抗癌作用、预防和治疗作用。方法：选择MCF-7细胞作为模型癌细胞。采用MTT法评价OMAE对细胞生长的体外抑制作用。此外，采用实时荧光定量PCR （qRT-PCR）检测与癌症相关的特定基因，如ESR1、Bax、Bcl-2和p53。此外，使用彗星测定法评估DNA损伤。结果：OMAE对MCF-7作用48 h后细胞增殖的IC50为53.1，IC90为97.5 μ g/ml。此外，ESR1基因在MCF-7细胞系中的表达水平显著降低。此外，与未处理的对照细胞（9.5%）相比，OMAE处理后的彗星长度和彗星阳性细胞（88.7%）显著增加，表明OMAE对DNA损伤的诱导程度很高。此外，OMAE还能改变bcl-2、肿瘤抑制基因（p53）和Bax水平，并通过释放细胞色素c影响Bax / bcl-2比值。结论：本研究结果是有希望的，提示本研究降低MCF7乳腺癌细胞凋亡率与OMAE可能具有潜在的抗癌作用有关，OMAE可能是一种合适的防癌饮品。然而，需要进一步的研究来充分了解OMAE作为癌症治疗的潜力。
Background: Chemotherapeutic agents have numerous side effects. There is a major interest in using natural and safe plants as food or drink to prevent from cancer. Origanum marjoram (OMAE) is a medicinal plant that can be used as a tea, food, and additive in traditional medicine.Objective: This study aimed to evaluate the potential anticancer effects of OMAE as a soft drink for daily use against a model cancer, prevention and treatment.Method: MCF-7 cells were chosen as model cancer cells. The MTT assay was used to assess the in vitro inhibitory effects of OMAE on cell growth. Moreover, quantitative real-time PCR (qRT-PCR) was used to detect specific genes associated with cancer, such as ESR1, Bax, Bcl-2, and p53. Furthermore, the DNA damage was evaluated using the comet assay.Results: OMAE has IC50 of 53.1 and IC90 of 97.5 mu g/ml dependent inhibition of cell proliferation after 48 h of treatment toward MCF-7. Also, a significant decrease in the expression level of the ESR1 gene in the MCF-7 cell line. Furthermore, there was a significant increase in the comet length and comet-positive cells after treatment with OMAE (88.7%) compared with those in the untreated control cells (9.5%), suggesting a high induction of DNA damage by OMAE. Also, OMAE showed a modification in bcl-2, tumor suppressor gene (p53), and Bax levels and influenced the BAX/BCL-2 ratio via releasing the cytochrome C.Conclusion: The results of the study were promising, suggesting that the reduced apoptotic rate of MCF7 breast cancer cells in this work was correlated to the potential anticancer effect of OMAE which would be a suitable preventable drink against cancer. However, further studies are needed to fully understand the potential of OMAE as a cancer treatment.