The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
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pH 响应胶束对肝癌细胞同时传递 siRNA 和 QD
DOI：
10.3389/fphar.2019.01194
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发表时间：
2019-10-10
影响因子：
5.6
通讯作者：
Xu, Gaixia
中科院分区：
文献类型：
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作者：
Cao, Zhonglin;Xiao, Huiyu;Xu, Gaixia
最近，RNA干扰（RNAI）已成为癌症治疗的有前途的方法。但是，由于缺乏安全有效的载体，RNAi在诊所中的应用仍受到阻碍。在这项研究中，开发了基于多碳酸二酰基 - 二酰基 - 聚二酰基 - 甲基氨基 - 二甲基氨基）甲基丙烯酸乙酯（PCL-PDEM）阳离子共聚物的pH响应性胶束，以携带短的干扰RNA（siRNA），用于沉默的hepatoma inshepatoma in Hepatoma in Hepatoma中的沉默癌细胞。 PCL-PDEM-SIRNA/量子点（QDS）纳米LOPLEX的转染效率已达到约70％，IL-8的表达水平降低了约63％。此外，已经实现了QD和siRNA的代码传递，这有助于可视化siRNA递送过程。尚未观察到纳米颗粒的大量细胞毒性，这表明我们的反应性阳离子胶束在肝癌癌症治疗的临床试验中潜在。
Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.