HEPARIN - A CHIRAL MOBILE-PHASE ADDITIVE FOR CAPILLARY ZONE ELECTROPHORESIS
HEPARIN - A CHIRAL MOBILE-PHASE ADDITIVE FOR CAPILLARY ZONE ELECTROPHORESIS
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DOI：
10.1021/ac00091a011
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发表时间：
1994-10-01
影响因子：
7.4
通讯作者：
AGYEI, NM
中科院分区：
文献类型：
--
作者：
STALCUP, AM;AGYEI, NM
肝素是一种天然存在的多分散聚阴离子糖胺聚糖，被研究作为毛细管区带电泳的手性选择剂。获得了多种未衍生化药物（包括抗疟药和抗组胺药）的基线分离。在 10 mM 磷酸盐缓冲液中使用 2% 肝素 (w/w)，在 15 kV 外加电压下，在 pH 4.5 或 5 下进行分析。所有成功解析的溶质均含有至少两个氮，其中一个氮并入杂环芳香环中。此外，成功的对映拆分似乎要求分子中的氮彼此之间有一定的距离。结果表明，除了静电相互作用之外，溶质大小也可能在肝素手性识别机制中发挥作用。
Heparin, a naturally occuring, polydisperse, polyanionic glycosaminoglycan, was investigated as a chiral selector for capillary zone electrophoresis. Baseline separations were obtained for a variety of underivatized drugs including antimalarials and antihistamines. Analysis was carried out at a pH of 4.5 or 5 using 2% heparin (w/w) in a 10 mM phosphate buffer under an applied voltage of 15 kV. All of the solutes successfully resolved contained at least two nitrogens with one of the nitrogens incorporated in a heterocyclic aromatic ring. Further, successful enantioresolution seemed to require that the nitrogens be somewhat distant from each other in the molecule. The results suggest that, in addition to electrostatic interactions, solute size may also play a role in the heparin chiral recognition mechanism.