IL-1 beta release from cultured bronchial epithelial cells and bronchoalveolar lavage cells from allergic and normal humans following segmental challenge with ragweed
IL-1 beta release from cultured bronchial epithelial cells and bronchoalveolar lavage cells from allergic and normal humans following segmental challenge with ragweed
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DOI：
10.1006/cyto.1996.0097
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发表时间：
1996-09-01
期刊：
影响因子：
3.8
通讯作者：
Peters, SP
中科院分区：
文献类型：
--
作者：
Hastie, AT;Everts, KB;Peters, SP
这项研究调查了白细胞介素1 β从上皮细胞释放的IL-1 β通过体内抗原攻击和体外炎性细胞调节。在过敏和正常个体中在节段性变应原攻击之前和之后获得支气管上皮细胞，并在有和没有自体支气管肺泡灌洗（BAL）细胞的情况下培养，用酶联免疫吸附法（ELISA）测定培养液中IL-1 β的含量。（4.4 ± 0.8 pg/ml，n = 32）和正常（6.8 ± 1.7 pg/ml，n = 17）受试者。在两个受试者组中，与没有共培养的BAL细胞共培养相比，上皮细胞中的IL-1 β水平显著升高（过敏，13.2 +/- 2.3 pg/ml，P = 0.006;正常：16.4 +/- 4.0 pg/ml，P = 0.007）。两组中来自无BAL细胞的上皮细胞的激发后IL-1 β均增加，但仅对过敏受试者具有显著性（P = 0.003）。对于过敏受试者，来自具有BAL细胞的上皮细胞的激发后IL-1 β变化很小（13.8 +/- 2.4 pg/ml），而对于正常受试者则增加（20.0 +/- 4.8 pg/ml）。肿瘤坏死因子α产生减少在过敏受试者中观察到TNF-α（第1天：447 pg/ml vs第2天：258 pg/ml），此外，激发前上皮+ BAL细胞释放的TNF-α和IL-1 β水平与过敏性相关性良好（r = 0.84）与正常人（r = 0.6），但激发后释放仅在正常受试者中相关这些结果表明支气管上皮细胞和BAL细胞相互作用，调节这些炎性细胞因子的释放。(C)1996年学术出版社
This work investigated whether interleukin 1 beta (IL-1 beta) release from epithelial cells is modulated by antigen challenge in vivo, and inflammatory cells in vitro, Bronchial epithelial cells were obtained before and after segmental allergen challenge in allergic and normal individuals, and were cultured with and without autologous bronchoalveolar lavage (BAL) cells, IL-1 beta in culture medium was quantitated by enzyme-linked immunoabsorbent assay (ELISA), Pre-challenge IL-1 beta levels from epithelial cells were similar in allergic (4.4 +/- 0.8 pg/ml, n = 32) and normal (6.8 +/- 1.7 pg/ml, n = 17) subjects. IL-1 beta levels were significantly elevated from epithelium with BAL cell co-culture vs without co-culture in both subject groups (allergic, 13.2 +/- 2.3 pg/ml, P = 0.006; normal: 16.4 +/- 4.0 pg/ml, P = 0.007). Post-challenge IL-1 beta from epithelial cells without BAL cells was increased in both groups, but significant only for allergic subjects (P = 0.003). Post-challenge IL-1 beta from epithelial with BAL cells changed little for allergic subjects (13.8 +/- 2.4 pg/ml), and increased for normal subjects (20.0 +/- 4.8 pg/ml). Decreased production of tumour necrosis factor alpha (TNF-alpha) was observed in allergic subjects (day 1: 447 pg/ml vs day 2: 258 pg/ml), Moreover, pre challenge release of TNF-alpha and IL-1 beta levels from epithelial + BAL cells correlated well for allergic (r = 0.84) and normal subjects (r = 0.6), but post-challenge release correlated only in normal subjects (r = 0.90), These results indicate that bronchial epithelial cells and BAL cells interact, modulating release of these inflammatory cytokines. (C) 1996 Academic Press Limited