Automation in High-Content Flow Cytometry Screening
Automation in High-Content Flow Cytometry Screening
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DOI：
10.1002/cyto.a.20754
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发表时间：
2009-09-01
期刊：
影响因子：
3.7
通讯作者：
Wand, M. P.
中科院分区：
文献类型：
--
作者：
Naumann, U.;Wand, M. P.
高含量流式细胞术筛选（FC-HCS）是一项21世纪世纪的技术，它结合了机器人流体处理、流式细胞术仪器和生物信息学软件，因此可以在短时间内处理和分析相对大量的流式细胞术样本。我们回顾了最近应用FC-HCS的问题，急性移植物抗宿主病的细胞签名定义。我们的重点是利用统计方法的最新进展实现数据处理步骤的自动化。我们证明，有效的结果，与通过手动处理获得的，可以实现使用我们的自动化技术。FC-HCS的这种自动化具有极大地改善诊断和生物标志物鉴定的潜力。(C)2009年国际细胞计数促进学会
High-content flow cytometric screening (FC-HCS) is a 21st Century technology that combines robotic fluid handling, flow cytometric instrumentation, and bioinformatics software, so that relatively large numbers of flow cytometric samples can be processed and analysed in a short period of time. We revisit a recent application of FC-HCS to the problem of cellular signature definition for acute graft-versus-host-disease. Our focus is on automation of the data processing steps using recent advances in statistical methodology. We demonstrate that effective results, on par with those obtained via manual processing, can be achieved using our automatic techniques. Such automation of FC-HCS has the potential to drastically improve diagnosis and biomarker identification. (C) 2009 International Society for Advancement of Cytometry