Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells.
Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells.
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DOI：
10.1016/j.jmbbm.2012.10.001
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发表时间：
2013-01
影响因子：
3.9
通讯作者：
Soker S
中科院分区：
文献类型：
--
作者：
Skardal A;Mack D;Atala A;Soker S
由干细胞所在的基质提供给干细胞的物理线索已被证明在调节干细胞的行为中起着至关重要的作用。直到最近，大多数研究都集中在基质弹性对分化能力的影响，而不是维持长期的增殖和可塑性。本研究的主要目的是研究羊水干细胞与不同弹性生长基质的相互作用，以拓展其应用潜力。在这里，我们研究了弹性模量（E′）对AFS细胞增殖、形态、细胞表面标志物表达和细胞迁移的自分泌刺激的影响。在不同E′底物上培养的AFS细胞增殖和形态发生明显变化。免疫组化显示，在较软的基质上培养的细胞中，与间充质干细胞（MSC）相关的细胞表面标志物（CD 44、CD 90、CD 105和N-钙粘蛋白）表达增加。此外，在较软的基质上培养的AFS细胞诱导自分泌刺激迁移。因此，将生物材料的弹性模量定制为特定的刚度值是控制干细胞性质的有效方法，这可以调节其治疗应用的有效性。
The physical cues presented to stem cells by the substrate on or in which they exist have been shown to play a crucial role in regulation of their behavior. Until recently, most research has focused on the effects of substrate elasticity on differentiation capability rather than maintenance of long-term proliferation and plasticity. The main goal of the present study is to study the interaction of amniotic fluid-derived stem (AFS) cells with growth substrata of different elasticity, which may extend their application potentials. Here, we investigate the effects of elastic modulus (E′), on AFS cell proliferation, morphology, cell surface marker expression, and autocrine stimulation of cell migration. AFS cells cultured on substrates of different E′ exhibited significant changes in proliferation and morphology. Immunohistochemistry revealed increased expression of cell surface markers associated with mesenchymal stem cells (MSCs) (CD44, CD90, CD105, and N-cadherin) in cells cultured on softer substrates. Additionally, AFS cells cultured on softer substrates induced autocrine stimulation of migration. Therefore, tailoring the elastic modulus of biomaterials to specific stiffness values is an effective method to control stem cell properties, which may modulate the effectiveness of their therapeutic applications.