IMPROVED FREE-ENERGY PARAMETERS FOR PREDICTIONS OF RNA DUPLEX STABILITY
IMPROVED FREE-ENERGY PARAMETERS FOR PREDICTIONS OF RNA DUPLEX STABILITY
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DOI：
10.1073/pnas.83.24.9373
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
TURNER, DH
中科院分区：
文献类型：
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作者：
FREIER, SM;KIERZEK, R;TURNER, DH
报道了用于预测RNA双链稳定性的热力学参数。从45个RNA寡核苷酸双链形成螺旋的焓变和自由能变化推导出了一个双链起始参数和10个螺旋延伸参数。选择寡聚物序列是为了使二级结构预测的可靠性最大化。在45个寡核苷酸中，10种最近邻序列中的每一种都有很好的代表性，并且选择这些序列是为了使37℃时ΔG⁰的实验误差最小化。这些参数预测大多数寡核苷酸双链的解链温度在5℃以内。这与最近邻模型所能预期的效果差不多。还列出了悬垂末端、末端错配和内部G - U错配时螺旋延伸的自由能变化，以及发夹环、内部环或内部凸起处螺旋起始的自由能变化。
Thermodynamic parameters for prediction of RNA duplex stability are reported. One parameter for duplex initiation and 10 parameters for helix propagation are derived from enthalpy and free-energy changes for helix formation by 45 RNA oligonucleotide duplexes. The oligomer sequences were chosen to maximize reliability of secondary structure predictions. Each of the 10 nearest-neighbor sequences is well-represented among the 45 oligonucleotides, and the sequences were chosen to minimize experimental errors in .DELTA.G0 at 37.degree. C. These parameters predict melting temperatures of most oligonucleotide duplexes within 5.degree. C. This is about as good as can be expected from the nearest-neighbor model. Free-energy changes for helix propagation at dangling ends, terminal mismatches, and internal G-U mismatches, and free-energy changes for helix initiation at hairpin loops, internal loops, or internal bulges are also tabulated.