p65 fragments, homologous to the C2 region of protein kinase C, bind to the intracellular receptors for protein kinase C.
p65 fragments, homologous to the C2 region of protein kinase C, bind to the intracellular receptors for protein kinase C.
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p65 片段与蛋白激酶 C 的 C2 区同源，与蛋白激酶 C 的细胞内受体结合。
DOI：
10.1021/bi00150a003
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Smith,BL
中科院分区：
文献类型：
--
作者：
Mochly-Rosen,D;Miller,KG;Scheller,RH;Khaner,H;Lopez,J;Smith,BL
1992年5月20日收到的摘要：活化蛋白激酶C（RACK）的受体已从心脏和大脑的颗粒细胞部分中分离出来。我们以前证明，结合蛋白激酶C（PKC）的RACK需要PKC激活剂，是通过一个网站上的PKC是从底物结合位点不同。在这里，我们研究的可能性，在PKC的调节结构域的C2区域参与结合PKC RACKs。突触囊泡特异性p65蛋白含有与PKC的C2区同源的两个区域。我们发现，三个p65片段，含有一个或两个这些PKC C2同源区，结合到高度纯化的RACK。p65片段和PKC与RACKs的结合是相互排斥的; RACKs与p65片段预孵育抑制PKC结合，RACKs与PKC预孵育抑制p65片段的结合。p65片段与RACKs上类似于PKC结合位点的肽预孵育也抑制p65与RACKs的结合，表明PKC和p65结合到RACKs上的相同或附近区域。由于PKC和p65片段之间的唯一同源区域是C2区，这些结果表明PKC上的C2区至少包含部分RACK结合位点。
Revised Manuscript Received May 20, 1992 abstract: Receptors for activated protein kinase C (RACKs) have been isolated from the particulate cell fraction of heart and brain. We previously demonstrated that binding of protein kinase C (PKC) to RACKs requires PKC activators and is via a site on PKC that is distinct from the substrate binding site. Here, we examine the possibility that the C2 region in the regulatory domain of PKC is involved in binding of PKC to RACKs. The synaptic vesicle-specificp65 protein contains two regions homologous to the C2 region of PKC. We found that three p65 fragments, containing either one or two of these PKC C2 homologous regions, bound tohighly purified RACKs. Binding of the p65 fragments and PKC to RACKs was mutually exclusive; preincubation of RACKs with the p65 fragmentsinhibited PKC binding, and preincubation of RACKs with PKC inhibited binding of the p65 fragments. Preincubation of the p65 fragments with a peptide resembling the PKC binding site on RACKs also inhibited p65 binding to RACKs, suggesting that PKC and p65 bind to the same or nearby regions on RACKs. Since the only homologous region between PKC and the p65 fragments is the C2 region, these results suggest that the C2 region on PKC contains at least part of the RACK binding site.