GROWTH COMPLEMENTATION OF INFLUENZA-VIRUS TEMPERATURE-SENSITIVE MUTANTS IN MOUSE CELLS WHICH EXPRESS THE RNA-POLYMERASE AND NUCLEOPROTEIN GENES
GROWTH COMPLEMENTATION OF INFLUENZA-VIRUS TEMPERATURE-SENSITIVE MUTANTS IN MOUSE CELLS WHICH EXPRESS THE RNA-POLYMERASE AND NUCLEOPROTEIN GENES
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DOI：
10.1093/oxfordjournals.jbchem.a123592
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发表时间：
1991-09-01
影响因子：
2.7
通讯作者：
NAKADA, S
中科院分区：
文献类型：
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作者：
NAKAMURA, Y;ODA, K;NAKADA, S
为了建立补充流感病毒温度敏感 (ts) 突变体生长的细胞系，将分别与小鼠乳腺肿瘤病毒 LTR 连接的三个 RNA 聚合酶和核蛋白 (NP) 基因克隆到牛乳头状瘤病毒载体 DNA 中。用这些重组质粒共转染小鼠C127细胞后，建立了细胞系克隆76，其中三种聚合酶和NP基因的表达可以被地塞米松刺激。克隆76细胞可以在非允许温度下补充聚合酶亚基基因之一缺陷的ts突变体的生长，以响应地塞米松。结果表明，为了有效补充 ts 突变体的生长，需要在蛋白质合成机器的同一区室中同时表达三种聚合酶基因。
In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papilloma-virus vector DNA. After co-transfection of mouse C127 cells with these recombinant plasmids, a cell line, clone 76, in which the expression of the three polymerase and NP genes could be stimulated by dexamethasone, was established. The clone 76 cells could complement the growth of ts-mutants defective in one of the polymerase subunit genes at the nonpermissive temperature in response to dexamethasone. The results suggest that the simultaneous expression of the three polymerase genes in the same compartment of protein synthesis machinery is required for an efficient complementation of ts-mutant growth.