Integrated self-regulating resistive heating for isothermal nucleic acid amplification tests (NAAT) in Lab-on-a-Chip (LoC) devices
Integrated self-regulating resistive heating for isothermal nucleic acid amplification tests (NAAT) in Lab-on-a-Chip (LoC) devices
复制标题
DOI：
10.1371/journal.pone.0189968
复制
发表时间：
2017-12-21
期刊：
影响因子：
3.7
通讯作者：
Stewart, Ray
中科院分区：
文献类型：
--
作者：
Pardy, Tamas;Tulp, Indrek;Stewart, Ray
芯片实验室（LoC）形式的恒温核酸扩增测试（NAAT）有望将高准确度、非仪器化的快速测试带到护理点。可靠的传染病快速检测有助于早期诊断和治疗，从而更好地遏制潜在的爆发，减少并发症。LoC NAAT的关键组件是加热元件，因为所有NAAT方案都需要在高温下孵育。我们提出了一种廉价的、集成的、自调节的电阻加热解决方案，该解决方案使用2xAAA碱性电池作为电源，可以将温度保持在60 +/- 63摄氏度范围内至少25分钟，并在5分钟内从室温达到目标范围。在LoC NAAT器械的热模型中评价了具有不同电气特性的4个加热元件样品。基于温度分布选择最佳加热元件候选。最佳的候选人进一步评估热建模通过有限元分析的热传递，并证明适合于等温核酸扩增。
Isothermal nucleic acid amplification tests (NAAT) in a Lab-on-a-Chip (LoC) format promise to bring high-accuracy, non-instrumented rapid tests to the point of care. Reliable rapid tests for infectious diseases allow for early diagnosis and treatment, which in turn enables better containment of potential outbreaks and fewer complications. A critical component to LoC NAATs is the heating element, as all NAAT protocols require incubation at elevated temperatures. We propose a cheap, integrated, self-regulating resistive heating solution that uses 2xAAA alkaline batteries as the power source, can maintain temperatures in the 60 +/- 63 degrees C range for at least 25 minutes, and reaches the target range from room temperature in 5 minutes. 4 heating element samples with different electrical characteristics were evaluated in a thermal mock-up for a LoC NAAT device. An optimal heating element candidate was chosen based on temperature profiling. The optimal candidate was further evaluated by thermal modelling via finite element analysis of heat transfer and demonstrated suitable for isothermal nucleic acid amplification.