Evaluation of Antimalarial Resistance Marker Polymorphism in Returned Migrant Workers in China
Evaluation of Antimalarial Resistance Marker Polymorphism in Returned Migrant Workers in China
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DOI：
10.1128/aac.04144-14
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Xia, Zhigui
中科院分区：
文献类型：
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作者：
Feng, Jun;Li, Jun;Xia, Zhigui
由于疟疾本地传播病例的减少和国际间的频繁交流，输入性疟疾已成为中国公共卫生面临的巨大挑战。恶性疟原虫是造成日益严重影响的主要原因。目前，青蒿琥酯加阿莫地喹是世界卫生组织推荐的青蒿素联合疗法之一，在中国主要用于治疗无并发症的恶性疟原虫疟疾。然而，在返乡农民工中的耐药标志物多态性尚未得到证实。在这里，我们评估了pfmdr1和pfcrt多态性的患病率，以及K13螺旋桨基因，青蒿素耐药的分子标记，在从加纳返回到上林县，广西省，中国，在2013年。共随机选择118份血液样本用于测定。在11个分离株中发现pfmdr1基因的突变，覆盖密码子86，184，1034和1246。N86Y突变频率最高（9.7%），其中Y86Y184S1034D1246突变频率最高（63.6%）。pfcrt基因的突变位点覆盖密码子74、75和76，在17个菌株中观察到，M74 N75 T76在三种单倍型中常见（70.6%）。在10个中国样本中首次观察到K13螺旋桨的8种不同基因型，2种同义突变（V487V和A627A）和6种非同义突变。C580Y在所有样本中最常见（2.7%）。本研究结果有助于丰富抗疟药耐药性的分子监测，并为我国抗疟指南的制定和更新提供参考。
Imported malaria has been a great challenge for public health in China due to decreased locally transmitted cases and frequent exchange worldwide. Plasmodium falciparum has been mainly responsible for the increasing impact. Currently, artesunate plus amodiaquine, one of the artemisinin combination therapies recommended by the World Health Organization, has been mainly used against uncomplicated P. falciparum malaria in China. However, drug resistance marker polymorphism in returning migrant workers has not been demonstrated. Here, we have evaluated the prevalence of pfmdr1 and pfcrt polymorphisms, as well as the K13 propeller gene, a molecular marker of artemisinin resistance, in migrant workers returned from Ghana to Shanglin County, Guangxi Province, China, in 2013. A total of 118 blood samples were randomly selected and used for the assay. Mutations of the pfmdr1 gene that covered codons 86, 184, 1034, and 1246 were found in 11 isolates. Mutations at codon N86Y (9.7%) were more frequent than at others, and Y86Y184S1034D1246 was the most prevalent (63.6%) of the four haplotypes. Mutations of the pfcrt gene that covered codons 74, 75, and 76 were observed in 17 isolates, and M74N75T76 was common (70.6%) in three haplotypes. Eight different genotypes of the K13 propeller were first observed in 10 samples in China, 2 synonymous mutations (V487V and A627A) and 6 nonsynonymous mutations. C580Y was the most prevalent (2.7%) in all the samples. The data presented might be helpful for enrichment of molecular surveillance of antimalarial resistance and will be useful for developing and updating antimalarial guidance in China.