High Resolution Cell Positioning Based on a Flow Reduction Mechanism for Enhancing Deformability Mapping
High Resolution Cell Positioning Based on a Flow Reduction Mechanism for Enhancing Deformability Mapping
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DOI：
10.3390/mi5041188
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发表时间：
2014-12-01
期刊：
影响因子：
3.4
通讯作者：
Kaneko, Makoto
中科院分区：
文献类型：
--
作者：
Sakuma, Shinya;Kuroda, Keisuke;Kaneko, Makoto
细胞变形性图的离散度不仅受传感系统分辨率的影响，而且受细胞变形性本身的影响。为了提取细胞的纯变形特性，有必要提高传感系统中细胞致动的分辨率，特别是在主动传感的情况下，其中致动器是必不可少的。本文提出了一种新的概念，“流量减少机制”，其中流动是由放置在微流控芯片外部的宏致动器产生的。由于嵌入微流体系统的流体回路中的弹性，在微通道中的细胞操作点处的流动可以急剧减少。这种方法的巨大优点是，我们可以通过组合宏观尺度致动器和宏观尺度位置传感器来容易地构建高分辨率细胞操作系统，即使致动器的分辨率大于细胞操作的期望分辨率。针对这一特点，我们成功地实现了基于视觉反馈控制的细胞定位，分辨率为240 nm，对应于视觉系统的一个像素。我们表明，利用这种定位系统有助于减少来自细胞变形性映射中的定位分辨率的分散。
The dispersion of cell deformability mapping is a ffected not only by the resolution of the sensing system, but also by cell deformability itself. In order to extract the pure deformability characteristics of cells, it is necessary to improve the resolution of cell actuation in the sensing system, particularly in the case of active sensing, where an actuator is essential. This paper proposes a novel concept, a "flow reduction mechanism", where a flow is generated by a macroactuator placed outside of a microfluidic chip. The flow can be drastically reduced at the cell manipulation point in a microchannel due to the elasticity embedded into the fluid circuit of the microfluidic system. The great advantage of this approach is that we can easily construct a high resolution cell manipulation system by combining a macro-scale actuator and a macro- scale position sensor, even though the resolution of the actuator is larger than the desired resolution for cell manipulation. Focusing on this characteristic, we successfully achieved the cell positioning based on a visual feedback control with a resolution of 240 nm, corresponding to one pixel of the vision system. We show that the utilization of this positioning system contributes to reducing the dispersion coming from the positioning resolution in the cell deformability mapping.