Computed tomography of amyloid plaques in a mouse model of Alzheimer's disease using diffraction enhanced imaging.
Computed tomography of amyloid plaques in a mouse model of Alzheimer's disease using diffraction enhanced imaging.
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DOI：
10.1016/j.neuroimage.2009.03.019
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发表时间：
2009-07-15
期刊：
影响因子：
5.7
通讯作者：
Zhong Z
中科院分区：
文献类型：
--
作者：
Connor DM;Benveniste H;Dilmanian FA;Kritzer MF;Miller LM;Zhong Z
我们对阿尔茨海默病（AD）早期发展的理解受到疾病进展规模的影响；淀粉样蛋白β (a β)斑块是阿尔茨海默病的一个标志性特征，在临床诊断成像技术中，斑块很小（~ 50 μm），对比度低。衍射增强成像（DEI）是一种相衬x射线成像技术，比传统的放射照相具有更大的软组织对比度，产生的图像分辨率比磁共振微成像更高。因此，在这项原理验证研究中，我们在微ct模式下对ad模型小鼠的大脑进行了DEI，以确定DEI是否可以显示Aβ斑块。结果显示，在大脑皮层和海马小结节。组织学证实，DEI图像显示的特征是Aβ斑块。一些解剖结构，包括海马亚区和白质轨道，也被观察到。因此，DEI在阿尔茨海默病的早期诊断以及小鼠大脑的一般研究中具有很强的前景。
Our understanding of early development in Alzheimer's disease (AD) is clouded by the scale at which the disease progresses; amyloid beta (Aβ) plaques, a hallmark feature of AD, are small (∼50 μm) and low contrast in diagnostic clinical imaging techniques. Diffraction enhanced imaging (DEI), a phase contrast x-ray imaging technique, has greater soft tissue contrast than conventional radiography and generates higher resolution images than magnetic resonance microimaging. Thus, in this proof of principle study, DEI in micro-CT mode was performed on the brains of AD-model mice to determine if DEI can visualize Aβ plaques. Results revealed small nodules in the cortex and hippocampus of the brain. Histology confirmed that the features seen in the DEI images of the brain were Aβ plaques. Several anatomical structures, including hippocampal subregions and white matter tracks, were also observed. Thus, DEI has strong promise in early diagnosis of AD, as well as general studies of the mouse brain.