Identification of an autocrine mechanism for regulating cell-cycle progression in murine keratinocytes.
Identification of an autocrine mechanism for regulating cell-cycle progression in murine keratinocytes.
复制标题
鉴定调节小鼠角质形成细胞细胞周期进程的自分泌机制。
DOI：
10.1007/bf01269884
复制
发表时间：
1997
影响因子：
3.6
通讯作者：
Slaga,TJ
中科院分区：
文献类型：
--
作者：
Curtin,GM;Fischer,SM;Slaga,TJ
Primary murine keratinocytes possess a limited doubling potential regardless of plating density or the inclusion of competence factors insulin, epidermal growth factor, and/or fetal bovine serum within the culture medium. In contrast, a murine cell line (CH-72), derived from a 7,12-dimethylbenz[a]-anthraceneinitiated, 12-O-tetra-decanoylphorbol-13-acetate-promoted mouse skin carcinoma, was found to exhibit unlimited proliferative potential; this was demonstrated by the ability of these cells to produce the progression factor required for entry into the DNA-synthesis phase in the absence of competence-factor stimulation. Conditioned medium, collected from murine carcinoma cells, was subsequently shown to increase the level of [3H] thymidine incorporation in competence-factor-deprived CH-72 cultures by more than a factor of 4 within 16h. Moreover, consistent with its ability of recruit cells cycling within the first gap phase directly into the DNA-synthesis phase, the autocrine progression factor present in conditioned medium decreased the G1 ∶ S ratio from the 55 ∶ 29 observed with growth medium controls to 38 ∶ 46. Preliminary characterization of the autocrine factor produced by cultured murine carcinoma cells using gel-filtration chromatography revealed a molecular mass of less than 2 kDa, similar in size to the factor previously shown by our laboratory to promote G1-phase progression in cultures of normal human foreskin keratinocytes.
