Competition between citrate and heptapeptide DDSDEEN binding to DNA in presence of divalent cations
Competition between citrate and heptapeptide DDSDEEN binding to DNA in presence of divalent cations
复制标题
DOI：
10.1023/a:1011955013990
复制
发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Marsili, V
中科院分区：
文献类型：
--
作者：
Calzuola, I;Castigli, E;Marsili, V
比较了柠檬酸和酸性肽DDSDEEN在二价阳离子存在下与DNA的结合。与肽相比，柠檬酸在DNA上显示出更多的结合位点；这可能是由于与柠檬酸相比，肽在DNA凹槽中的结合具有更大的电阻值。此外，用饱和量的柠檬酸预孵育的DNA不能与连续添加的肽结合。因此肽和柠檬酸盐结合位点在一定程度上重叠。
The binding of citrate and acidic peptide DDSDEEN with DNA in the presence of divalent cations is compared. Citric acid shows a higher number of binding sites on the DNA compared to the peptide; this is probably due to the bigger sitric hindrance of the peptide compared to the citric acid for the binding in the DNA grooves. Moreover, DNA preincubated with saturating amounts of citric acid is not available for the binding with successively added peptide. Therefore the peptide and citrate binding sites to some extent overlap.