Canine Population Data Generated from a Multiplex STR Kit for Use in Forensic Casework
Canine Population Data Generated from a Multiplex STR Kit for Use in Forensic Casework
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DOI：
10.1111/j.1556-4029.2009.01080.x
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发表时间：
2009-07-01
影响因子：
1.6
通讯作者：
Koskinen, Mikko T.
中科院分区：
文献类型：
--
作者：
Kanthaswamy, Sree;Tom, Bradley K.;Koskinen, Mikko T.
犬类生物标本通常是犯罪现场物证的一部分。到目前为止，还没有经过验证的犬类专用法医试剂盒可用。建立了一个由18个短串联重复序列(STR)和一个性别连锁的锌指基因座组成的多重基因分型系统，用于产生评估犬法医DNA图谱重量的群体遗传学数据。对居住在美国的236只纯种犬和431只混血犬的等位基因频率进行了估计。使用地区数据库的平均随机匹配概率为1/2x10(33)，使用品种数据集的平均随机匹配概率为1/4x10(39)。每个家系群体在遗传上是不同的，可以从混血犬群体中区分出来，但遗传变异与地理转变没有显着的相关性。结果支持使用等位基因频率数据与犬的STR多重，以传达身份测试的意义，法医案件，亲子鉴定，和品种分配。
Canine biological specimens are often part of the physical evidence from crime scenes. Until now, there have been no validated canine-specific forensic reagent kits available. A multiplex genotyping system, comprising 18 short tandem repeats (STRs) and a sex-linked zinc finger locus for gender determination, was developed for generating population genetic data assessing the weight of canine forensic DNA profiles. Allele frequencies were estimated for 236 pedigreed and 431 mixed breed dogs residing in the U.S. Average random match probability is 1 in 2 x 10(33) using the regional database and 1 in 4 x 10(39) using the breed dataset. Each pedigreed population was genetically distinct and could be differentiated from the mixed breed dog population but genetic variation was not significantly correlated with geographic transition. Results herein support the use of the allele frequency data with the canine STR multiplex for conveying the significance of identity testing for forensic casework, parentage testing, and breed assignments.