Novel culture and exposure system for measurement of effects of airborne pollutants on mammalian cells
Novel culture and exposure system for measurement of effects of airborne pollutants on mammalian cells
复制标题
用于测量空气污染物对哺乳动物细胞影响的新型培养和暴露系统
DOI：
--
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
T. Crocker
中科院分区：
文献类型：
--
作者：
G. Samuelsen;R. Rasmussen;B. Nair;T. Crocker
作者：Samuelsen，GS；拉斯穆森，RE；奈尔，BK；克罗克，TT |摘要：描述了一种在类似于体内的条件下将体外哺乳动物细胞暴露于空气污染物的新方法。通过从过滤器的另一表面灌注营养培养基，在膜过滤器上生长的细胞在暴露期间得到滋养并防止干燥。细胞被一层薄薄的液体覆盖，其厚度与覆盖呼吸道上皮的粘液层相当。接触城市环境空气中通常测量的二氧化氮 (0.1-0.2 ppm) 浓度，在接触污染物不到 4 小时后，就会产生明显的细胞毒性作用。建议该细胞暴露系统可用于研究空气污染物对哺乳动物细胞的影响。 © 1978，美国化学会。版权所有。
Author(s): Samuelsen, GS; Rasmussen, RE; Nair, BK; Crocker, TT | Abstract: A novel method for exposing mammalian cells in vitro to air pollutants under conditions resembling those in vivo is described. Cells grown on membrane filters are nourished and protected from drying during exposure by perfusion of nutrient medium from the opposite surface of the filter. The cells are covered by a thin layer of fluid comparable in thickness to the mucus layer overlying the respiratory epithelium. Exposure to NO2 at levels commonly measured in the ambient air of urban settings (0.1-0.2 ppm) produces a pronounced cytotoxic effect after less than 4 h of pollutant encounter. It is proposed that this cell exposure system will have utility in the study of effects of airborne pollutants on mammalian cells. © 1978, American Chemical Society. All rights reserved.