Transcriptional Regulation of the Ethylene Response Factor LeERF2 in the Expression of Ethylene Biosynthesis Genes Controls Ethylene Production in Tomato and Tobacco
Transcriptional Regulation of the Ethylene Response Factor LeERF2 in the Expression of Ethylene Biosynthesis Genes Controls Ethylene Production in Tomato and Tobacco
复制标题
乙烯生物合成基因表达中乙烯反应因子 LeERF2 的转录调控控制番茄和烟草中的乙烯生产
DOI：
10.1104/pp.109.135830
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发表时间：
2009-05-01
期刊：
影响因子：
7.4
通讯作者：
Huang, Rongfeng
中科院分区：
文献类型：
--
作者：
Zhang, Zhijin;Zhang, Haiwen;Huang, Rongfeng
乙烯产生的微调在发育过程和植物对胁迫的反应中起着重要的作用，但对乙烯生物合成基因和乙烯产生中的乙烯反应因子(ERF)蛋白的调节知之甚少。因此，确定在这一过程中发挥作用的顺式作用元件和转录因子是重要的。在此之前，番茄(Solanum Lycopsicum[f.sp.Erf蛋白，LeERF2，TERF2的等位基因，被报道在植物上赋予乙烯三重反应。本文报道了LeERF2/TERF2在番茄和烟草乙烯生物合成中的转录调控。利用高表达和反义LeERF2/TERF2转基因番茄，我们发现LeERF2/TERF2是乙烯生物合成基因表达和乙烯产生的重要调节因子。表达分析表明，LeERF2/TERF2是乙烯诱导的，并且在反义LeERF2/TERF2转基因番茄中，乙烯刺激的乙烯产生受到抑制，这表明LeERF2/TERF2在乙烯诱导的反馈环中是一个正调控因子。进一步的研究表明，LeERF2/TERF2保守地调控烟草乙烯的生物合成，这种调节在烟草中与下胚轴的伸长以及发芽和幼苗发育过程中对脱落酸和葡萄糖不敏感有关。对乙烯合成的影响与另一种ERF蛋白TERF1相似，因为TERF1和LeERF2/TERF2在烟草乙烯生物合成的转录调控中具有重叠的作用。生化分析表明，LeERF2/TERF2在NtACS3启动子中与GCC盒相互作用，在LeACO3启动子中与脱水反应元件相互作用，导致番茄和烟草乙烯生物合成相关基因的转录激活，这是ERF蛋白在植物乙烯生物合成中的一种新的调控功能。
Fine-tuning of ethylene production plays an important role in developmental processes and in plant responses to stress, but very little is known about the regulation of ethylene response factor (ERF) proteins in ethylene biosynthesis genes and ethylene production. Identifying cis-acting elements and transcription factors that play a role in this process, therefore, is important. Previously, a tomato (Solanum lycopersicum [f. sp. Lycopersicon esculentum]) ERF protein, LeERF2, an allele of TERF2, was reported to confer ethylene triple response on plants. This paper reports the transcriptional modulation of LeERF2/TERF2 in ethylene biosynthesis in tomato and tobacco (Nicotiana tabacum). Using overexpressing and antisense LeERF2/TERF2 transgenic tomato, we found that LeERF2/TERF2 is an important regulator in the expression of ethylene biosynthesis genes and the production of ethylene. Expression analysis revealed that LeERF2/TERF2 is ethylene inducible, and ethylene production stimulated by ethylene was suppressed in antisense LeERF2/TERF2 transgenic tomato, indicating LeERF2/TERF2 to be a positive regulator in the feedback loop of ethylene induction. Further research showed that LeERF2/TERF2 conservatively modulates ethylene biosynthesis in tobacco and that such regulation in tobacco is associated with the elongation of the hypocotyl and insensitivity to abscisic acid and glucose during germination and seedling development. The effects on ethylene synthesis were similar to those of another ERF protein, TERF1, because TERF1 and LeERF2/TERF2 have overlapping roles in the transcriptional regulation of ethylene biosynthesis in tobacco. Biochemical analysis showed that LeERF2/TERF2 interacted with GCC box in the promoter of NtACS3 and with dehydration-responsive element in the promoter of LeACO3, resulting in transcriptional activation of the genes for ethylene biosynthesis in tomato and tobacco, which is a novel regulatory function of ERF proteins in plant ethylene biosynthesis.