Respiration of arsenate and selenate by hyperthermophilic archaea
Respiration of arsenate and selenate by hyperthermophilic archaea
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DOI：
10.1016/s0723-2020(00)80058-2
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发表时间：
2000-10-01
影响因子：
3.4
通讯作者：
Rose, D
中科院分区：
文献类型：
--
作者：
Huber, R;Sacher, M;Rose, D
从意大利那不勒斯Pisciarelli Solfatara的一个小屋泉中分离出一种严格厌氧、极端嗜热、兼性有机营养古菌。杆状细胞以二氧化碳为碳源，氢为电子供体，砷酸盐、硫代硫酸盐或单质硫为电子受体，进行化能自养生长。H2S由硫或硫代硫酸盐生成，亚砷酸盐由砷酸盐生成。有机营养，新的分离物生长最佳的无机电子受体，如硫，硒酸盐或砷酸盐的存在下。培养，生长在砷酸盐和硫代硫酸盐：或砷酸盐和L-半胱氨酸，沉淀雄黄（As 2S 2）。在硒酸盐上生长期间，产生元素硒。DNA的G+C含量为58.3mol%。根据16 S rRNA基因序列分析结合生理和形态学标准，新分离物属于热蛋白目。它代表了一个新的物种在Pyrobaculum属，其中的模式种，我们命名为Pyrobaculum arsenaticum（模式菌株PZ 6 *，DSM 13514，ATCC 700994）。与不同的Pyrobaculum物种的比较研究表明，Pyrobaculum aerophilum也能够生长在厌氧培养条件下，在砷酸盐，硒酸盐和亚硒酸盐的存在下有机营养。在亚硒酸盐上生长期间，形成元素硒作为最终产物。与P、Arsenaticum相比，P. aerophilum可以利用Seenate或Arsenate进行二氧化碳和氢气的岩石自养生长。
A novel, strictly anaerobic, hyperthermophilic, facultative organotrophic archaeon was isolated from a hut spring at Pisciarelli Solfatara, Naples, Italy. The rod-shaped cells grew chemolithoautotrophically with carbon dioxide as carbon source, hydrogen as electron donor and arsenate, thiosulfate or elemental sulfur as electron acceptor. H2S was formed from sulfur or thiosulfate, arsenite from arsenate. Organotrophically, the new isolate grew optimally in the presence of an inorganic electron acceptor like sulfur, selenate or arsenate. Cultures, grown on arsenate and thiosulfate: or arsenate and L-cysteine, precipitated realgar (As2S2). During growth on selenate, elemental selenium was produced. The G+C content of the DNA was 58.3 mol%. Due to 16S rRNA gene sequence analysis combined with physiological and morphological criteria, the new isolate belongs to the Thermoproteales order. It represents a new species within the genus Pyrobaculum, the type species of which we name Pyrobaculum arsenaticum (type strain PZ6*, DSM 13514, ATCC 700994).Comparative studies with different Pyrobaculum-species showed, that Pyrobaculum aerophilum was also able to grow organotrophically under anaerobic culture conditions in the presence of arsenate, selenate and selenite. During growth on selenite, elemental selenium was formed as final product. In contrast to P, arsenaticum, P. aerophilum could use selenate or arsenate for lithoautotrophic growth with carbon dioxide and hydrogen.